The product under consideration is High Quality 3,3′-diaminobenzidine with the CAS number 91 - 95 - 2. It is a chemical compound known for its specific molecular structure and chemical properties. The molecular formula of 3,3′-diaminobenzidine is C₁₂H₁₄N₄. It typically appears as a solid powder with a certain color, often light - colored depending on its purity and manufacturing process.
Regarding its purity, our product is of high - quality, meeting strict industry standards. The high purity ensures its reliability and effectiveness in various applications. Its melting point and boiling point are important physical parameters that play a role in its handling and storage. The melting point is in a specific range which can be provided upon request, and the boiling point also falls within a defined interval.
3,3′-diaminobenzidine has a wide range of applications in different fields. In the biological research area, it is commonly used as a chromogenic substrate in immunohistochemistry. When combined with specific enzymes, it can produce a visible color reaction, allowing researchers to visualize the location of specific proteins or antigens in tissue samples. This is crucial for understanding biological processes, diagnosing diseases, and developing new drugs.
In the field of material science, 3,3′-diaminobenzidine can be used in the synthesis of certain polymers. Its reactive amino groups can participate in polymerization reactions, leading to the formation of polymers with unique properties such as good mechanical strength, heat resistance, and chemical stability. These polymers can be used in the manufacture of high - performance materials, such as aerospace components, electronic devices, and automotive parts.
When using 3,3′-diaminobenzidine in immunohistochemistry, first, prepare a suitable buffer solution to dissolve the compound. The concentration of the solution needs to be carefully adjusted according to the experimental requirements. Usually, a low - concentration solution is used for preliminary experiments, and then the concentration can be optimized based on the results.
After preparing the solution, apply it to the tissue section. The application process should be carried out under a controlled environment to avoid contamination. Incubate the tissue section with the 3,3′-diaminobenzidine solution for a certain period of time, which is determined by the specific experimental protocol. Finally, wash the section thoroughly to remove any unreacted compound and observe the color reaction under a microscope.
In polymer synthesis, 3,3′-diaminobenzidine should be added to the reaction system at the appropriate stage. The reaction conditions, such as temperature, pressure, and the presence of catalysts, need to be carefully controlled to ensure the success of the polymerization reaction. Appropriate safety measures, such as wearing protective gloves and goggles, should be taken during the handling process.
Case 1: A research team in a medical laboratory was studying a specific cancer - related protein. They used 3,3′-diaminobenzidine in an immunohistochemical experiment. First, they prepared a 0.5 mg/ml solution of 3,3′-diaminobenzidine in a Tris - HCl buffer. Tissue samples from cancer patients were obtained and processed. The 3,3′-diaminobenzidine solution was then applied to the tissue sections, and after a 15 - minute incubation period, a clear brown color reaction was observed in the areas where the target protein was present. This result helped the researchers to accurately locate the distribution of the cancer - related protein in the tissue, which is valuable for further research on cancer diagnosis and treatment.
Case 2: A materials research group was working on developing a new type of heat - resistant polymer. They added 3,3′-diaminobenzidine to a reaction mixture containing other monomers. The reaction was carried out at a temperature of 150°C under nitrogen atmosphere for 5 hours. After purification, the resulting polymer showed excellent heat resistance, with a glass - transition temperature of over 200°C. This polymer has potential applications in the production of high - temperature - resistant components in the aerospace industry.
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