1. Product Information

Product Name: Spermidine Trihydrochloride

CAS Number: 334-50-9

Molecular Formula: C₇H₁₉N₃·3HCl

Molecular Weight: 254.6 g/mol

Appearance: White crystalline powder

Purity: ≥98% (HPLC)

Storage: Store at -20°C in a dry, dark environment

2. Product Usage

Spermidine Trihydrochloride (CAS 334-50-9) is a polyamine compound widely used in biochemical and pharmaceutical research. Key applications include:

• Inducing autophagy in cellular studies
• Anti-aging and longevity research
• Neurodegenerative disease studies (e.g., Alzheimer’s, Parkinson’s)
• Enzyme activity modulation in vitro
• Cell culture medium supplementation

3. How to Use

Step-by-Step Guide:

1. Storage: Keep sealed at -20°C; avoid repeated freeze-thaw cycles.
2. Preparation: Dissolve in sterile PBS or cell culture-grade water to desired concentration (typical working concentration: 1-10 µM).
3. Application: Add to cell culture media or experimental buffers. Optimize dosage based on cell type and assay requirements.
4. Safety: Use gloves and eye protection; avoid inhalation or direct contact.

4. Application Cases

Case 1: Autophagy Induction in HEK293 Cells

Researchers used 5 µM Spermidine Trihydrochloride in DMEM media for 24 hours, resulting in a 40% increase in LC3-II/LC3-I ratio, confirming autophagy activation.

Case 2: Neuroprotection in Mouse Models

Daily intraperitoneal injection of 10 mg/kg Spermidine Trihydrochloride for 14 days reduced amyloid-beta plaque formation by 30% in Alzheimer’s disease models.

Case 3: In Vitro Enzyme Kinetics

Added 2 mM Spermidine Trihydrochloride to reaction buffers, enhancing acetyltransferase activity by 25% in kinetic assays.

5. Contact Information

For technical inquiries or bulk orders:

• Email: info@vivalr.com
• Phone: (86) 15866781826