Factory Supply Giemsa Stain CAS 51811 - 82 - 6 With Low Price User Guide

1. Basic Information and Parameters
2. Product Uses
3. Product Usage
4. Usage Case Studies
5. Contact Information

Basic Information and Parameters

Giemsa stain with the CAS number 51811 - 82 - 6 is a well - known histological and cytological stain. This product is factory - supplied, which ensures a stable quality and low price. It is a mixture of methylene blue, eosin, and other dyes, which gives it unique staining properties.

The stain is usually available in liquid form. The pH of the working solution is typically around 6.8 - 7.2 to ensure optimal staining results. It has a specific density that is consistent with its formulation, which helps in accurate preparation of staining solutions. The product is sensitive to light and should be stored in a cool, dark place to maintain its efficacy.

Product Uses

1. In parasitology, Giemsa stain is widely used to detect various parasitic infections. It can clearly stain the parasites such as Plasmodium (the causative agent of malaria) in blood smears. By staining, the parasites can be easily identified under a microscope, which is crucial for the diagnosis and treatment of malaria.

2. In hematology, it is used for staining blood cells. It can differentiate different types of white blood cells, such as neutrophils, lymphocytes, monocytes, eosinophils, and basophils. This differentiation helps in the diagnosis of blood - related diseases, like leukemia and anemia.

3. In cytogenetics, Giemsa stain is used for chromosome banding. The characteristic banding patterns on chromosomes can be observed after staining, which is helpful for genetic analysis, including the detection of chromosomal abnormalities.

Product Usage

1. Preparation of the working solution: Dilute the stock solution of Giemsa stain according to the manufacturer's instructions. Usually, a 1:20 to 1:50 dilution with a buffer solution (such as phosphate buffer) is appropriate. The buffer solution should be at the correct pH to ensure good staining.

2. Fixation of the sample: For blood smears or tissue sections, they need to be fixed first. Methanol is commonly used as a fixative. Immerse the sample in methanol for a few minutes to ensure proper fixation.

3. Staining process: Place the fixed sample in the working solution of Giemsa stain. The staining time can vary depending on the sample type and the desired staining intensity. Generally, it takes 10 - 30 minutes. After staining, rinse the sample gently with distilled water to remove the excess stain.

4. Observation: After staining and rinsing, let the sample dry completely. Then, observe it under a microscope. Use appropriate magnification to clearly see the stained structures.

Usage Case Studies

Case 1: Malaria Diagnosis
A hospital in a malaria - endemic area received a patient with symptoms of fever and chills. A blood smear was prepared from the patient's blood sample. The smear was fixed with methanol and then stained with Giemsa stain according to the standard protocol. Under the microscope, the characteristic ring - shaped trophozoites of Plasmodium falciparum were clearly visible. This diagnosis led to the timely treatment of the patient with antimalarial drugs, which improved the patient's condition.

Case 2: Leukemia Detection
A patient with fatigue and abnormal blood cell counts visited the hospital. A blood sample was taken, and the blood cells were stained with Giemsa stain. The staining revealed abnormal white blood cell morphology, with a large number of immature cells. Further examination and analysis based on the stained sample confirmed the diagnosis of acute lymphoblastic leukemia. This early detection allowed for prompt initiation of chemotherapy, which is crucial for the patient's prognosis.

Contact Information

If you have any questions about our Factory Supply Giemsa Stain CAS 51811 - 82 - 6, please feel free to contact us. Our email address is info@vivalr.com, and our phone number is (86) 15866781826.