Our product, High Quality Guanidine hydrochloride with CAS 50 - 01 - 1, is a chemical compound known for its high purity and excellent quality. The chemical formula of guanidine hydrochloride is CH₅N₃·HCl. It appears as a white crystalline powder. This product is carefully manufactured under strict quality control measures to ensure its high quality and stability. The purity of our guanidine hydrochloride is typically above 99%, which meets the high - end requirements of various industries. It has a melting point in the range of 172 - 176°C and is highly soluble in water, methanol, and ethanol. The density of this compound is around 1.354 g/cm³ at 25°C.
1. **Protein Denaturation**: Guanidine hydrochloride is a powerful protein denaturant. In biochemical research, it is widely used to disrupt the non - covalent bonds in proteins, unfolding them into their primary structure. This is crucial for protein purification, sequencing, and studying protein folding pathways. For example, in proteomics research, it helps in the isolation and analysis of individual proteins from complex biological samples.
2. **Nucleic Acid Extraction**: It can also be used in nucleic acid extraction protocols. By denaturing proteins associated with nucleic acids, it facilitates the separation of DNA or RNA from other cellular components, ensuring high - quality nucleic acid samples for downstream applications such as PCR, sequencing, and gene cloning.
3. **Pharmaceutical Industry**: In the pharmaceutical field, guanidine hydrochloride can be used as an intermediate in the synthesis of certain drugs. It participates in chemical reactions to form new compounds with potential therapeutic effects.
1. **Preparation of Solutions**: When using guanidine hydrochloride, it is usually dissolved in an appropriate solvent. For protein denaturation, a common approach is to prepare a stock solution with a concentration of 6 - 8 M in water. To do this, carefully weigh the required amount of guanidine hydrochloride and slowly add it to the solvent while stirring continuously. After dissolution, the solution can be filtered to remove any insoluble impurities.
2. **Protein Denaturation Process**: Add the prepared guanidine hydrochloride solution to the protein sample at an appropriate ratio. Incubate the mixture at a suitable temperature (usually around room temperature or 37°C) for a specific period of time to ensure complete denaturation. The incubation time depends on the nature of the protein and the concentration of the denaturant.
3. **Nucleic Acid Extraction**: In nucleic acid extraction, guanidine hydrochloride is often used in combination with other reagents. First, lyse the cells or tissues in a buffer containing guanidine hydrochloride. Then, perform a series of extraction and purification steps, such as phenol - chloroform extraction and ethanol precipitation, to obtain the pure nucleic acid.
1. **Protein Purification in a Research Lab**: A research team was studying a novel protein involved in a specific disease pathway. They used guanidine hydrochloride to denature the protein from a complex cell lysate. First, they prepared an 8 M guanidine hydrochloride solution. They added this solution to the cell lysate at a ratio of 1:1 and incubated the mixture at 37°C for 2 hours. After that, they used chromatography techniques to purify the denatured protein. The high - quality guanidine hydrochloride ensured complete denaturation, which made the subsequent purification process more efficient. Finally, they were able to obtain a pure protein sample for further structural and functional studies.
2. **Nucleic Acid Extraction in a Diagnostic Lab**: In a diagnostic laboratory, they needed to extract high - quality DNA from clinical samples for pathogen detection. They used a guanidine hydrochloride - based nucleic acid extraction kit. The guanidine hydrochloride in the kit effectively denatured the proteins in the samples, allowing for the release and purification of DNA. By following the standard protocol provided with the kit, they were able to obtain high - yield and high - purity DNA samples, which were then successfully used in PCR - based pathogen detection assays.
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