1. Descripción general del producto

MOPSO (3-(N-Morpholino)-2-hydroxypropanesulfonic acid) is a high-quality biological buffer with CAS Number 68399-77-9. Las especificaciones clave incluyen:

• Fórmula molecular: C₇H₁₅NO₅S
• Peso molecular: 249.26 g/mol
• Apariencia: Polvo cristalino blanco
• Pureza: ≥99% (HPLC)
• pH Range: 6.5-7.9
• Almacenamiento: 2-8°C, dry environment

2. Applications of MOPSO Buffer

MOPSO is widely used in biochemical and molecular biology research due to its stable pH performance and low toxicity. Key applications include:

• Cell culture media preparation
• Protein purification and stabilization
• Enzyme activity assays
• Electrophoresis and chromatography
• Diagnostic reagent formulations

3. How to Use MOPSO Buffer

Guía paso a paso:

1. Dissolve MOPSO powder in distilled water at 20-25°C (recommended concentration: 0.1-0.2 M).
2. Adjust pH using NaOH or HCl while monitoring with a calibrated pH meter.
3. Filter-sterilize (0.22 µm) for cell culture applications.
4. Store prepared buffer at 4°C for short-term use or -20°C for long-term storage.

Nota: Avoid contact with strong oxidizers and heavy metal ions.

4. Casos de uso detallados

Case 1: Mammalian Cell Culture

MOPSO (20 mM) was used to maintain pH stability in HEK293 cell cultures at 37°C/5% CO₂. The buffer showed no cytotoxicity after 72 hours of incubation.

Caso 2: Purificación de proteínas

In a His-tag protein purification workflow, 50 mM MOPSO (pH 7.4) with 300 mM NaCl provided optimal binding conditions for Ni-NTA resin, achieving 95% purity.

5. 5. Información de contacto

For technical support or bulk orders of MOPSO (CAS 68399-77-9):

• Correo electrónico: info@vivalr.com
• Teléfono: (86) 15866781826