Deoxythymidine triphosphate (dTTP), with the CAS number 18423 - 43 - 3, is a crucial nucleotide in the field of molecular biology. Our product offers high - quality dTTP at a low price, meeting the needs of various research and industrial applications.
The molecular formula of dTTP is C₁₀H₁₄N₂O₁₃P₃, and its molecular weight is approximately 482.13 g/mol. It exists as a white to off - white powder in its solid form. Under standard conditions, it has good stability, but it should be stored properly to maintain its activity. It is recommended to store dTTP at - 20°C to prevent degradation and ensure its long - term usability.
Deoxythymidine triphosphate plays a vital role in DNA synthesis. In polymerase chain reaction (PCR), dTTP is one of the four essential deoxynucleotide triphosphates (dNTPs). PCR is a widely used technique for amplifying specific DNA sequences. During the PCR process, DNA polymerase incorporates dTTP into the newly synthesized DNA strand according to the complementary base - pairing rule, allowing for the exponential amplification of the target DNA.
It is also used in DNA sequencing. Sanger sequencing, a classic DNA sequencing method, relies on the incorporation of modified dNTPs (including dTTP) to determine the nucleotide sequence of a DNA fragment. In modern next - generation sequencing technologies, dTTP is still an indispensable component in the DNA synthesis step of the sequencing reaction.
Moreover, dTTP is used in DNA labeling experiments. By incorporating labeled dTTP (such as biotin - labeled or fluorescent - labeled dTTP) into DNA, researchers can track and detect specific DNA molecules in various biological samples.
When using dTTP, first, ensure that you are working in a clean and sterile environment to prevent contamination. Thaw the dTTP stock solution at room temperature or on ice. Once thawed, gently vortex the solution to ensure uniform mixing.
In PCR reactions, the typical concentration of dTTP in the reaction mixture is usually in the range of 20 - 200 μM. The exact concentration may need to be optimized according to the specific PCR protocol and the target DNA sequence. Add the appropriate volume of dTTP stock solution to the PCR reaction mix along with other components such as primers, DNA template, DNA polymerase, and buffer.
For DNA sequencing and labeling experiments, follow the specific protocols provided by the sequencing or labeling kits. Generally, the amount of dTTP used is adjusted according to the scale of the experiment and the requirements of the reaction system.
Case 1: PCR Amplification of a Specific Gene
A research team was studying a gene related to a certain disease. They used our high - quality dTTP in their PCR experiments. They designed specific primers for the target gene and set up a PCR reaction system with a dTTP concentration of 100 μM. After optimizing the PCR conditions, they successfully amplified the target gene fragment. The amplified product was then analyzed by gel electrophoresis, showing a clear and specific band, indicating the high efficiency and reliability of our dTTP in PCR applications.
Case 2: Sanger Sequencing of a Bacterial Genome
A microbiology laboratory was sequencing the genome of a newly isolated bacterium. They used our dTTP in their Sanger sequencing reactions. By carefully following the sequencing protocol and using the appropriate amount of dTTP, they obtained high - quality sequencing data. The sequence information helped them to understand the genetic characteristics of the bacterium, such as the presence of potential virulence genes and antibiotic - resistance genes.
If you have any questions or need further assistance regarding our High Quality Deoxythymidine triphosphate CAS 18423 - 43 - 3 With Low Price, please feel free to contact us.
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