The product is High Quality 2′-Deoxyguanosine-5′-triphosphate Trisodium Salt with the CAS number 93919 - 41 - 6. This compound is an important nucleotide derivative. It has a specific chemical structure that plays a crucial role in biological and biochemical processes. The “2′-Deoxyguanosine” part indicates its base - guanine combined with a deoxyribose sugar, and the “5′-triphosphate” means it has three phosphate groups attached to the 5′ position of the sugar. The trisodium salt form enhances its solubility and stability in aqueous solutions. In terms of quality, it is of high - grade, ensuring reliable performance in various applications. The product is also offered at the best price, providing cost - effective solutions for users.
This product has a wide range of applications in the fields of molecular biology and biotechnology. In DNA synthesis, it serves as a building block. During polymerase chain reaction (PCR), 2′-Deoxyguanosine-5′-triphosphate is one of the four essential deoxynucleotide triphosphates (dNTPs). DNA polymerases incorporate these dNTPs into the growing DNA strand, using the template DNA to guide the correct sequence. In gene cloning, it is used to create recombinant DNA molecules. Scientists can use it to amplify specific DNA fragments, which are then inserted into vectors for further manipulation and study. It is also used in DNA sequencing techniques, such as Sanger sequencing and next - generation sequencing. The accurate incorporation of this nucleotide is crucial for obtaining reliable sequencing results.
When using 2′-Deoxyguanosine-5′-triphosphate Trisodium Salt, proper handling and storage are essential. First, it should be stored at - 20°C in a dry and dark place to maintain its stability. Before use, it should be thawed slowly on ice to prevent degradation. When preparing reaction mixtures, such as for PCR, the appropriate concentration of the product needs to be added according to the experimental protocol. Usually, the final concentration of dNTPs in a standard PCR reaction is around 200 μM. It is important to mix the reaction components thoroughly to ensure uniform distribution. When pipetting, use calibrated pipettes to ensure accurate volume measurement. After the reaction is set up, it should be placed in a thermocycler or other appropriate equipment for the reaction to proceed under the specified temperature and time conditions.
Case 1: PCR for Gene Amplification
A research team was interested in amplifying a specific gene fragment from a genomic DNA sample. They prepared a PCR reaction mixture containing the template genomic DNA, primers specific to the target gene, DNA polymerase, buffer, and the High Quality 2′-Deoxyguanosine-5′-triphosphate Trisodium Salt along with other dNTPs. They set the thermocycler with an initial denaturation step at 95°C for 5 minutes, followed by 30 cycles of denaturation at 95°C for 30 seconds, annealing at a suitable temperature for 30 seconds, and extension at 72°C for 1 minute. After the final extension step at 72°C for 10 minutes, they analyzed the PCR products by agarose gel electrophoresis. The result showed a clear and specific band corresponding to the target gene fragment, indicating the successful amplification using the product.
Case 2: DNA Sequencing
A biotechnology company was performing Sanger sequencing on a plasmid DNA. They used the product as one of the dNTPs in the sequencing reaction. The reaction mixture was prepared with the plasmid DNA template, sequencing primer, DNA polymerase, and the appropriate dNTPs including 2′-Deoxyguanosine-5′-triphosphate Trisodium Salt. The sequencing reaction was carried out according to the standard Sanger sequencing protocol. After the reaction, the DNA fragments were separated by capillary electrophoresis, and the sequencing results were obtained. The high - quality product ensured accurate base - calling and reliable sequencing data.
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