Factory Supply Sephadex LH20 is a product with the CAS number 9041 - 37 - 6. It is offered at a low price, which makes it an attractive option for various laboratories and research institutions. Sephadex LH20 is a cross - linked dextran gel that has been hydroxypropylated. This modification gives it unique physical and chemical properties.
Physically, it has a bead - like structure with a well - defined particle size distribution. The particle size typically ranges from 20 - 150 µm, which allows for efficient separation and purification processes. Chemically, it is stable in a wide range of solvents, including water, ethanol, methanol, and chloroform. Its molecular weight cut - off is in the range suitable for separating small to medium - sized molecules, usually from a few hundred to several thousand Daltons.
Sephadex LH20 has a wide range of applications in different fields. In the field of natural product research, it is commonly used for the isolation and purification of natural compounds such as alkaloids, flavonoids, and terpenoids. These compounds often have similar chemical structures, and Sephadex LH20 can effectively separate them based on their size and polarity differences.
In the pharmaceutical industry, it is used for the purification of drug intermediates and active pharmaceutical ingredients (APIs). By removing impurities and unwanted by - products, it helps to improve the quality and purity of the final pharmaceutical products. Additionally, in biochemical research, it can be used for the separation of peptides and small proteins, which is crucial for studying their structures and functions.
Before using Sephadex LH20, it is necessary to swell the gel. This can be done by suspending the dry gel in an appropriate solvent, such as ethanol or water, and allowing it to stand for several hours or overnight. The swollen gel should then be poured into a chromatography column. Care should be taken to avoid the formation of air bubbles, which can affect the separation efficiency.
The sample to be separated is dissolved in a suitable solvent and then loaded onto the top of the column. The eluent, which is a solvent or a mixture of solvents, is then passed through the column at a controlled flow rate. The separation is based on the different interactions between the sample components and the gel matrix. Components with weaker interactions will elute faster, while those with stronger interactions will elute later.
During the elution process, fractions are collected at regular intervals. These fractions can then be analyzed by various analytical techniques, such as high - performance liquid chromatography (HPLC) or nuclear magnetic resonance (NMR), to determine the presence and purity of the target components.
Case 1: In a natural product research project, a research team was trying to isolate a novel alkaloid from a plant extract. They used Sephadex LH20 in a chromatography column. After swelling the gel in ethanol and packing the column, they loaded the plant extract onto the column. By using a gradient elution with a mixture of ethanol and water, they were able to separate the target alkaloid from other impurities. The eluted fractions were analyzed by HPLC, and the purity of the isolated alkaloid was found to be over 95%. This pure alkaloid was then used for further pharmacological studies.
Case 2: A pharmaceutical company was working on the purification of a new drug intermediate. The intermediate was contaminated with several by - products. They employed Sephadex LH20 chromatography to purify the intermediate. After optimizing the elution conditions, they were able to obtain a highly pure drug intermediate, which met the quality requirements for further synthesis steps. This not only improved the yield of the final drug product but also reduced the production cost.
If you have any questions or need further information about Factory Supply Sephadex LH20 cas 9041 - 37 - 6, please feel free to contact us. Our email address is info@vivalr.com, and our phone number is (86) 15866781826.
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