The product, Factory Supply Nitrotetrazolium blue chloride with CAS number 298 - 83 - 9, is a chemical compound that is widely used in various scientific and industrial fields. It is known for its low price, which makes it an attractive option for many users.
Chemical Formula: C40H30Cl2N10O6
Molecular Weight: 817.64 g/mol
Appearance: Usually in the form of a yellowish - brown powder. It is soluble in some organic solvents such as dimethyl sulfoxide (DMSO) and ethanol.
Storage Conditions: It should be stored in a cool, dry place, away from direct sunlight and sources of heat. Keep the container tightly closed to prevent moisture and air from getting in, as these can potentially affect the stability of the compound.
1. Biological Research: Nitrotetrazolium blue chloride is commonly used as an indicator in biological assays. It can be reduced by certain enzymes or reducing agents to form a colored formazan product. This property is used to detect the presence and activity of enzymes such as dehydrogenase in cells and tissues. For example, in microbiology, it can be used to identify bacteria based on their enzymatic activity.
2. Histology: In histological studies, this compound can be used to stain tissues. The reduction of nitrotetrazolium blue chloride to formazan provides a visible marker, which helps researchers to observe and analyze specific cellular structures or metabolic processes in tissues.
3. Industrial Applications: In some industrial processes, it can be used as a redox indicator. Its ability to change color upon reduction makes it useful in monitoring chemical reactions where oxidation - reduction processes are involved.
1. Preparation of Solution: First, you need to prepare a suitable solution of nitrotetrazolium blue chloride. Weigh an appropriate amount of the powder according to your experimental requirements. For example, if you need a 1 mg/mL solution, dissolve 100 mg of the powder in 100 mL of the appropriate solvent (such as DMSO or ethanol). Stir the solution well until the powder is completely dissolved. You can use a magnetic stirrer for better mixing.
2. Experimental Setup: In a biological assay, add the prepared solution to the sample or reaction mixture. The amount of solution added depends on the specific protocol. For example, if you are performing an enzymatic assay, you may add a few microliters to milliliters of the solution, depending on the volume of the reaction system. Incubate the mixture at the appropriate temperature and for the specified time. For most biological assays, this may be at 37°C for a period of 30 minutes to a few hours.
3. Observation and Analysis: After the incubation period, observe the color change in the sample. The appearance of a colored formazan product indicates a positive reaction. You can use a spectrophotometer to quantitatively measure the absorbance of the solution at the appropriate wavelength (usually around 520 - 580 nm, depending on the specific formazan product formed). Compare the absorbance values with a standard curve to determine the concentration of the analyte or the activity of the enzyme.
Case 1: Enzyme Activity Detection in Bacteria
A research team was studying the dehydrogenase activity in different strains of bacteria. They prepared a nitrotetrazolium blue chloride solution with a concentration of 2 mg/mL in DMSO. They inoculated different bacterial strains into a growth medium containing the nitrotetrazolium blue chloride solution. After incubating the cultures at 37°C for 24 hours, they observed a color change from yellow - brown to purple in some cultures. By measuring the absorbance of the cultures at 540 nm, they were able to quantify the dehydrogenase activity in each strain. This information helped them to understand the metabolic characteristics of different bacterial strains and their potential applications in bioremediation processes.
Case 2: Histological Staining of Muscle Tissue
A histology laboratory was conducting a study on muscle tissue development. They used nitrotetrazolium blue chloride to stain cross - sections of muscle tissue. They prepared a 1 mg/mL solution of the compound in ethanol and incubated the tissue sections in the solution for 1 hour at room temperature. After staining
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