The product named "Factory Supply HEPPS CAS 16052-06-5 With Low Price" is a high - quality chemical compound. Its CAS number, 16052 - 06 - 5, is a unique identifier for this specific chemical in the scientific and industrial community. This indicates that it has been well - recognized and documented in relevant chemical databases.
Regarding its physical and chemical properties, detailed information can be obtained from the product's material safety data sheet (MSDS). Generally, it is expected to have specific melting and boiling points, solubility characteristics, and stability under different environmental conditions. The low - price feature makes it an attractive option for various industries looking to control costs without sacrificing quality.
HEPPS (4 - (2 - Hydroxyethyl) piperazine - 1 - propanesulfonic acid) is widely used in biochemical and biological research. It can be used as a buffer in cell culture media. Buffers are crucial in maintaining a stable pH environment, which is essential for the normal growth and metabolism of cells. In cell culture, small fluctuations in pH can have a significant impact on cell viability and function, and HEPPS helps to mitigate these changes.
Moreover, it is also used in protein purification processes. During protein purification, maintaining the appropriate pH is necessary to ensure the stability and activity of the target protein. HEPPS can play a vital role in creating an optimal pH condition for protein separation and purification.
When using HEPPS in cell culture media, first, you need to accurately weigh the appropriate amount of the product according to the experimental requirements. Dissolve it in deionized water or the appropriate solvent. Then, use a pH meter to adjust the pH of the solution to the desired value. Usually, a small amount of acid or base can be added drop - wise to fine - tune the pH.
In protein purification, HEPPS can be added to the purification buffer. Before use, ensure that the buffer is well - mixed and filtered to remove any impurities. When handling HEPPS, it is important to follow good laboratory practices, wear appropriate personal protective equipment such as gloves and goggles, and work in a well - ventilated area.
Case Study 1: A research group was culturing a particular type of cancer cells for drug - sensitivity testing. They used HEPPS as a buffer in the cell culture medium. By using HEPPS, they were able to maintain a stable pH of 7.4 in the medium throughout the culture period. As a result, the cancer cells showed normal growth characteristics, and the drug - sensitivity test results were more reliable. In contrast, when they used a different buffer in a previous experiment, the pH fluctuated, leading to abnormal cell growth and inconsistent test results.
Case Study 2: A biotechnology company was purifying a recombinant protein. They incorporated HEPPS into the purification buffer. The HEPPS - containing buffer helped to maintain the stability of the protein during the purification process. The yield of the purified protein was significantly higher compared to previous attempts without HEPPS, and the purity of the protein was also improved.
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