Anthrone, with the CAS number 90 - 44 - 8, is a well - known organic compound. Its molecular formula is C₁₄H₁₀O, and it has a molecular weight of approximately 194.23 g/mol. It appears as a yellow crystalline solid. It has a melting point range of 155 - 158 °C and is insoluble in water but soluble in organic solvents such as ethanol, ether, and chloroform. The purity of the factory - supplied anthrone is strictly controlled to ensure high - quality products that meet various industrial and research needs.
Anthrone has a wide range of applications. In the field of biochemistry, it is commonly used as a reagent for the determination of carbohydrates. The anthrone reaction is a well - established method for the quantitative analysis of various sugars. It reacts with carbohydrates in a sulfuric acid medium to produce a colored complex, which can be measured spectrophotometrically. This method is highly sensitive and is widely used in food analysis, clinical diagnostics, and plant physiology research.
In the pharmaceutical industry, anthrone derivatives have shown potential as anti - cancer and anti - inflammatory agents. Some research studies have explored the structure - activity relationships of anthrone - based compounds and their effects on biological systems. Additionally, in the area of materials science, anthrone can be used as a building block for the synthesis of novel organic materials with unique optical and electronic properties.
When using anthrone for carbohydrate analysis, the following steps are typically followed. First, prepare a suitable sample solution. The sample should be dissolved or diluted in an appropriate solvent. Then, add a specific amount of anthrone reagent, which is usually prepared by dissolving anthrone in concentrated sulfuric acid. The reaction mixture is then heated in a water bath for a certain period of time to ensure complete reaction. After cooling, the absorbance of the solution is measured at a specific wavelength (usually around 620 nm) using a spectrophotometer. The concentration of carbohydrates in the sample can be determined by comparing the absorbance with a standard curve.
For handling anthrone, it is important to wear appropriate protective equipment, such as gloves and goggles, as it is a potentially hazardous chemical. It should be stored in a cool, dry place away from direct sunlight and sources of ignition. When using it in a laboratory or industrial setting, follow all relevant safety protocols and regulations.
Case 1: Food Analysis
A food testing laboratory was tasked with determining the carbohydrate content in a new brand of energy bars. They used the anthrone method to analyze the samples. First, they ground the energy bars into a fine powder and extracted the carbohydrates using a suitable solvent. Then, they added the anthrone reagent and followed the reaction procedure. By comparing the absorbance of the sample with a standard curve prepared from known carbohydrate solutions, they were able to accurately determine the carbohydrate content in the energy bars. This information was crucial for providing nutritional information to consumers.
Case 2: Biotechnology Research
A research team was studying the carbohydrate metabolism in plants. They used anthrone to measure the changes in carbohydrate levels in plant tissues under different environmental conditions. They collected plant samples at regular intervals and analyzed them using the anthrone method. The results showed how the carbohydrate content in the plants varied with factors such as light intensity and temperature, which provided valuable insights into plant physiology and adaptation mechanisms.
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