1. Información sobre el producto

Nombre del producto: Glutamate Dehydrogenase (GDH)

Número CAS: 9029-12-3 / 9029-11-2

Fuente: Recombinant expression from microbial strains

Pureza: ≥95% (SDS-PAGE)

Activity: ≥200 units/mg protein

Almacenamiento: Stable at 2-8°C for 24 months; avoid freeze-thaw cycles

2. Aplicaciones y usos

Glutamate Dehydrogenase is a critical enzyme widely used in:

• Diagnostic Kits: Detection of ammonia levels in blood/plasma.
• Investigación bioquímica: Study of amino acid metabolism and enzyme kinetics.
• Biotechnology: NAD(P)H regeneration systems in industrial biocatalysis.
• Food Safety Testing: Quantification of glutamate in processed foods.

3. Instrucciones de uso

Protocolo paso a paso:

1. Reconstitución: Dilute lyophilized GDH in 0.1M phosphate buffer (pH 7.4).
2. Sample Preparation: Centrifuge biological samples at 10,000×g for 5 minutes.
3. Reaction Setup: Mix 50µL sample with 100µL assay buffer, 10µL NAD⁺ (2mM), and 10µL enzyme.
4. Medición: Monitor absorbance at 340nm for NADH formation (25°C, 5 minutes).
5. Calculation: Use extinction coefficient ε₃₄₀ = 6.22 mM⁻¹cm⁻¹ for quantification.

Nota: Optimize enzyme concentration based on substrate load.

4. Casos de uso detallados

Case Study 1: Blood Ammonia Detection

Aplicación: Diagnosing hepatic encephalopathy in clinical settings.

Procedimiento:

1. Add 10µL plasma to 200µL reaction mix (containing α-ketoglutarate and NADH).
2. Incubate at 37°C for 15 minutes.
3. Measure NADH depletion rate at 340nm.

Resultado: Linear range 10-500µM ammonia with 98% correlation to reference methods.

Case Study 2: Glutamate Biosensor Development

Aplicación: Real-time monitoring in fermentation processes.

Procedimiento:

1. Immobilize GDH on carbon nanotube electrodes.
2. Calibrate using 0.1-10mM glutamate standards.
3. Measure current change via NADH oxidation.

Resultado: 90% signal stability after 50 operational cycles.