1. Descripción general del producto

ACES (CAS 7365-82-4), chemically known as 2-[(2-Amino-2-oxoethyl)amino]ethanesulfonic acid, is a high-purity biological buffer widely used in biochemical and pharmaceutical research. Key specifications include:

• Fórmula molecular: C₄H₁₀N₂O₄S
• Peso molecular: 182.2 g/mol
• Apariencia: Polvo cristalino blanco
• Pureza: ≥99% (HPLC)
• Almacenamiento: 2-8°C in dry, dark conditions

2. Applications of ACES (CAS 7365-82-4)

ACES is a zwitterionic buffer ideal for:

• Biochemical assays requiring pH stability between 6.1–7.5
• Electrophoresis and cell culture media preparation
• Enzyme kinetics studies and protein purification
• Diagnostic reagent formulations

Its low toxicity, minimal metal ion binding, and compatibility with biological systems make it a preferred choice for sensitive applications.

3. Directrices de uso

Preparation of ACES Buffer Solution:

1. Dissolve 10 g of ACES (CAS 7365-82-4) in 1 L of deionized water.
2. Adjust pH to 6.8–7.2 using NaOH or HCl.
3. Filter sterilize (0.22 µm) for cell culture applications.
4. Store at 4°C for up to 4 weeks.

Precauciones de seguridad:

• Llevar guantes y gafas para evitar el contacto con la piel y los ojos.
• Eliminar los residuos de acuerdo con la normativa local.

4. Casos prácticos

Case Study 1: Cell Culture Medium

ACES buffer (20 mM, pH 7.0) was used to maintain pH stability in mammalian cell cultures, enhancing cell viability by 30% compared to traditional buffers.

Case Study 2: DNA Electrophoresis

A 40 mM ACES-based running buffer (pH 6.9) improved resolution of DNA fragments ≤500 bp in agarose gels, reducing smearing effects.

Case Study 3: Enzyme Activity Assay

ACES (50 mM, pH 7.2) preserved 95% of lactate dehydrogenase activity after 24 hours at 25°C, outperforming phosphate buffers.