1. Descripción general del producto

DL-O-Phosphoserine (CAS 17885-08-4) is a high-purity biochemical reagent widely used in research and industrial applications. Key specifications include:

• Fórmula química: C₃H₈NO₆P
• Peso molecular: 185.07 g/mol
• Apariencia: Polvo cristalino blanco
• Pureza: ≥98% (HPLC)
• Almacenamiento: Store at -20°C in a dry, dark environment

2. Applications of DL-O-Phosphoserine

This phosphorylated amino acid derivative is essential in:

• Investigación bioquímica: Studying protein phosphorylation, kinase/phosphatase activity, and signal transduction pathways.
• Desarrollo farmacéutico: Serving as a substrate or intermediate in drug discovery for metabolic disorders.
• Cell Culture: Enhancing cell viability in media formulations for mammalian cell lines.
• Diagnostic Kits: Acting as a stabilizer or component in enzymatic assay reagents.

3. Directrices de uso

Recommended Protocols:

1. Reconstitución: Dissolve in PBS or deionized water at 10 mM (pH 7.4).
2. Experimental Use: Add 1-100 µM to reaction mixtures; optimize concentration based on assay requirements.
3. Almacenamiento: Aliquot and store at -20°C; avoid freeze-thaw cycles.
4. Seguridad: Wear PPE (gloves, goggles) and work in a well-ventilated area.

4. Casos de uso detallados

Case Study 1: Kinase Activity Assay

Objetivo: Measure kinase activity using DL-O-Phosphoserine as a substrate.

1. Prepare 20 µL reaction buffer (50 mM Tris-HCl, 10 mM MgCl₂, 1 mM DTT).
2. Add 5 µL of 10 mM DL-O-Phosphoserine solution.
3. Initiate reaction with 0.1 µg/mL kinase enzyme at 30°C for 30 min.
4. Terminate with 10% TCA and quantify phosphorylation via HPLC.

Case Study 2: Cell Culture Supplementation

Protocolo: Add 2 mM DL-O-Phosphoserine to DMEM medium for HEK293 cell cultures, improving protein expression yields by 40% compared to controls.