Factory Supply TAPS CAS 29915-38-6 With Low Price: Comprehensive User Guide
Índice
1. Información sobre el producto
Nombre del producto: TAPS (CAS 29915-38-6)
Nombre químico: Tris(hydroxymethyl)methylaminopropanesulfonic acid
Fórmula molecular: C7H17NO6S
Peso molecular: 243.28 g/mol
Apariencia: Polvo cristalino blanco
Pureza: ≥99%
Almacenamiento: Room temperature, dry environment
2. Applications of TAPS
TAPS (CAS 29915-38-6) is a zwitterionic biological buffer widely used in:
- Biochemical research and molecular biology experiments
- Cell culture media preparation
- Diagnostic reagent formulations
- Fabricación farmacéutica
Its effective buffering range is pH 7.7–9.1, making it ideal for stabilizing pH in sensitive biological systems. TAPS also exhibits low binding affinity to metal ions, ensuring minimal interference in enzymatic reactions.
3. Directrices de uso
Pasos de preparación:
- Dissolve TAPS in distilled water at a concentration of 0.1–1.0 M.
- Adjust pH using NaOH or HCl as required.
- Filter-sterilize the solution (0.22 µm filter) for cell culture applications.
Precauciones:
- Store in a tightly sealed container to avoid moisture absorption.
- Wear protective gloves and goggles during handling.
- Test compatibility with other compounds before large-scale use.
4. Casos de uso detallados
Case 1: Cell Culture Media Preparation
Paso 1: Dissolve 22.33 g of TAPS in 800 mL distilled water.
Segundo paso: Adjust pH to 8.5 with 1M NaOH.
Tercer paso: Sterilize by filtration and add to DMEM medium at 25 mM final concentration.
Resultado: Stable pH maintenance during mammalian cell growth.
Case 2: Diagnostic Reagent Formulation
Paso 1: Prepare 0.5M TAPS solution (pH 9.0).
Segundo paso: Mix with enzyme substrates and stabilizers.
Resultado: Enhanced accuracy in alkaline phosphatase-based assays.
5. 5. Información de contacto
Para pedidos al por mayor o asistencia técnica:
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- Teléfono: (86) 15866781826