1. Información sobre el producto

Nombre del producto: Lewis A Antigen (CAS 21973-23-9)

Número CAS: 21973-23-9

Fórmula química: C₁₉H₃₁NO₁₄

Peso molecular: 473.45 g/mol

Pureza: >95% (HPLC)

Apariencia: Polvo blanco a blanquecino

Almacenamiento: Store at -20°C in a dry environment.

2. Aplicaciones

Lewis A Antigen (CAS 21973-23-9) is widely used in:

• Investigación biomédica: Study of blood group antigens and cellular interactions.
• Cancer Research: Investigation of tumor-associated antigens in carcinomas.
• Diagnostic Reagents: Key component in immunoassays for disease detection.
• Glycobiology: Analysis of carbohydrate-mediated biological processes.

3. Instrucciones de uso

Guía paso a paso:

1. Manipulación de almacenamiento: Keep lyophilized powder at -20°C; avoid repeated freeze-thaw cycles.
2. Reconstitución: Dissolve in PBS (pH 7.4) to desired concentration (e.g., 1 mg/mL).
3. Experimental Use: Dilute to working concentration (0.1-10 µg/mL) for ELISA, flow cytometry, or immunohistochemistry.
4. Seguridad: Use gloves and lab coats; avoid inhalation or direct contact.

4. Detailed Case Studies

Case Study 1: Blood Type Classification

Objetivo: Identify Lewis A Antigen expression in human erythrocytes.

Method:

1. Prepare 0.5 mg/mL Lewis A solution in PBS.
2. Incubate with blood samples at 37°C for 1 hour.
3. Analyze via flow cytometry; positive results indicate blood group A subtype.

Case Study 2: Cancer Biomarker Research

Objetivo: Detect Lewis A overexpression in gastric cancer cells.

Method:

1. Fix tissue sections with paraformaldehyde.
2. Apply 2 µg/mL Lewis A Antigen solution for 2 hours.
3. Use anti-Lewis A antibodies for immunohistochemical staining.