The product is Factory Supply Geneticin CAS 108321 - 42 - 2 With Low Price. Geneticin, also known as G418 sulfate, is an aminoglycoside antibiotic that inhibits protein synthesis in bacteria, yeast, higher plants, mammalian cells, protozoans, and helminths. The CAS number 108321 - 42 - 2 uniquely identifies this chemical compound. It is typically in the form of a white to off - white powder. The purity of our Factory Supply Geneticin is very high, which guarantees its effectiveness in various applications. It is stable under normal storage conditions but should be kept in a cool, dry place away from light.
Geneticin is widely used in molecular biology and cell culture research. In molecular biology, it is commonly used as a selective agent in gene transfer experiments. When a gene of interest is introduced into cells along with a resistance gene for Geneticin, cells that have successfully incorporated the foreign DNA can be selected by growing them in a medium containing Geneticin. Only cells that express the resistance gene will survive and grow, allowing researchers to isolate the transfected cells. In cell culture, Geneticin can be used to eliminate unwanted cell populations. For example, in co - culture systems, it can be used to kill non - transfected or non - resistant cells, leaving behind only the desired cell type.
1. Preparation: First, dissolve the Geneticin powder in sterile water to make a stock solution. The recommended concentration of the stock solution is usually 50 - 100 mg/mL. Filter - sterilize the solution using a 0.22 - μm filter to remove any potential contaminants.
2. Cell Treatment: Add the appropriate amount of the stock solution to the cell culture medium. The working concentration of Geneticin varies depending on the cell type. For most mammalian cell lines, the working concentration ranges from 200 - 1000 μg/mL. It is advisable to conduct a dose - response experiment to determine the optimal concentration for your specific cell line.
3. Incubation: After adding Geneticin to the medium, gently mix the medium to ensure uniform distribution of the antibiotic. Then, return the cells to the incubator under appropriate culture conditions. Monitor the cells regularly for signs of cell death and growth.
Case 1: In a cancer research project, scientists wanted to study the function of a specific gene in a particular cancer cell line. They used Geneticin - based selection to create stable cell lines that overexpressed the gene of interest. First, they transfected the cancer cells with a plasmid containing the gene and a Geneticin resistance gene. After that, they added Geneticin to the culture medium at a concentration of 800 μg/mL. Within a week, most of the non - transfected cells died, and the remaining cells were the ones that had successfully integrated the plasmid. These stable cell lines were then used for further functional studies, such as cell proliferation assays and gene expression analysis.
Case 2: A biotechnology company was developing a new cell - based vaccine. They used Geneticin to ensure the purity of their cell culture. In their production process, they had a mixed population of cells, including some non - productive cells. By adding Geneticin to the culture at a concentration of 500 μg/mL, they were able to eliminate the non - productive cells, resulting in a higher - quality cell culture for vaccine production.
If you have any questions or need further information about our Factory Supply Geneticin CAS 108321 - 42 - 2, please feel free to contact us. Our email is info@vivalr.com, and our phone number is (86) 15866781826.
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