High Quality Dithioerythritol Cas 6892-68-8 is a well - known chemical compound. Its chemical formula is C4H10O2S2, and its molecular weight is approximately 154.25 g/mol. This product is presented in a high - quality form with a low price, making it an attractive option for various industries. It is a white to off - white crystalline powder that has good solubility in water and some organic solvents. The melting point of Dithioerythritol is around 41 - 43 °C. It has high chemical stability under normal storage conditions, but it should be kept away from strong oxidizing agents and high - temperature environments.
Dithioerythritol has a wide range of applications in different fields. In the field of biochemistry, it is commonly used as a reducing agent. It can break disulfide bonds in proteins, which is crucial for protein purification, protein structure analysis, and enzyme activity studies. By reducing disulfide bonds, it helps to maintain the native state of proteins and prevent protein aggregation. In the pharmaceutical industry, Dithioerythritol can be used in the synthesis of certain drugs. It can participate in chemical reactions to form key intermediates, which are then used to produce various therapeutic agents. Additionally, in the field of cell culture, it can be added to the culture medium to maintain a reducing environment, which is beneficial for the growth and survival of some sensitive cells.
When using High Quality Dithioerythritol Cas 6892-68-8, it is necessary to follow proper procedures. First, wear appropriate personal protective equipment, such as gloves and safety glasses, to avoid direct contact with the compound. For biochemical experiments, dissolve the appropriate amount of Dithioerythritol in a suitable buffer solution. The concentration usually ranges from 1 mM to 10 mM, depending on the specific experiment requirements. Make sure to dissolve it completely by gentle stirring or vortexing. When adding it to a reaction system, add it slowly to ensure uniform mixing. In pharmaceutical synthesis, follow the specific reaction conditions and stoichiometry. Measure the required amount accurately using appropriate measuring tools. After use, store the remaining product in a tightly sealed container at a low - temperature environment to maintain its quality.
Case 1: Protein Purification
In a research laboratory, a team was trying to purify a specific protein from a complex biological sample. They added Dithioerythritol to the sample at a concentration of 5 mM. By breaking the disulfide bonds in the protein, it prevented the protein from aggregating and made it easier to separate from other impurities using chromatography techniques. As a result, they were able to obtain a high - purity protein sample, which was crucial for their subsequent protein structure and function studies.
Caso 2: Cultivo celular
A cell culture facility was culturing a type of primary cells that were very sensitive to oxidative stress. They added Dithioerythritol to the culture medium at a concentration of 2 mM. The reducing environment created by Dithioerythritol significantly improved the cell viability and growth rate. The cells showed better morphological characteristics and higher metabolic activity compared to the control group without Dithioerythritol.
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Este es William, CEO de Zhishang Chemical Co., Ltd.
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