The product is High Quality Collagenase with CAS number 9001-12-1. Collagenase is an enzyme that breaks down the peptide bonds in collagen. This high - quality collagenase is available at the best price, ensuring cost - effectiveness for users.
It is usually presented in a highly purified form, with a specific activity that can vary depending on the batch. The enzyme is stable under specific storage conditions. For long - term storage, it is recommended to keep it at - 20°C to maintain its activity. The purity of the product is carefully controlled to minimize impurities that could affect its performance.
1. **Biomedical Research**: In cell isolation, collagenase is used to dissociate tissues into single - cell suspensions. This is crucial for many research areas such as stem cell research, cancer research, and immunology. It helps researchers to study cell behavior in a more controlled environment.
2. **Pharmaceutical Industry**: In the development of drugs, collagenase can be used to study the degradation of extracellular matrix components. It also plays a role in the formulation of some topical medications for wound healing, as it can help break down the collagen - rich scar tissue.
3. **Food Industry**: Collagenase can be used in the processing of meat products to improve tenderness. By breaking down the collagen in muscle tissue, it can enhance the texture and palatability of the meat.
1. **Reconstitution**: First, reconstitute the lyophilized collagenase with an appropriate buffer. The choice of buffer depends on the specific application. Generally, a phosphate - buffered saline (PBS) or a Tris - based buffer can be used. Follow the instructions on the product label for the correct buffer and volume.
2. **Dilution**: After reconstitution, the collagenase solution may need to be diluted to the appropriate concentration. This concentration is determined by the type of tissue being processed and the desired outcome. For most tissue dissociation applications, a concentration ranging from 0.1 - 1 mg/ml is commonly used.
3. **Incubation**: Add the collagenase solution to the tissue sample and incubate at an appropriate temperature, usually 37°C, for a specific period of time. The incubation time can vary from 30 minutes to several hours, depending on the tissue type and the collagenase concentration used. Stirring or gentle shaking during incubation can improve the efficiency of the digestion process.
1. **Cancer Research**: A research team was studying the invasive properties of cancer cells. They used our high - quality collagenase to dissociate tumor tissues into single - cell suspensions. By treating the tumor tissues with a collagenase solution at a concentration of 0.5 mg/ml for 2 hours at 37°C, they were able to obtain a large number of viable cancer cells. These cells were then used for further in - vitro assays, which helped them to understand the mechanisms of cancer cell invasion and metastasis.
2. **Wound Healing Treatment**: A pharmaceutical company was developing a new topical wound - healing cream. They incorporated our collagenase into the cream formulation. In a clinical trial, patients with chronic wounds were treated with the cream. The collagenase in the cream helped to break down the excessive collagen in the scar tissue, promoting faster wound healing. After a few weeks of treatment, significant improvement in wound closure was observed in the patients.
If you have any questions or need further information about our High Quality Collagenase CAS 9001 - 12 - 1, please feel free to contact us:
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Este es William, CEO de Zhishang Chemical Co., Ltd.
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