The product, High Quality HEPES Sodium Salt with CAS 75277-39-3, is a high - grade chemical compound. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) sodium salt is a zwitterionic buffer widely used in biological and biochemical research. It has excellent buffering capacity in the pH range of 6.8 - 8.2, which makes it highly suitable for maintaining the stability of biological systems.
The purity of our HEPES sodium salt is extremely high, ensuring reliable and reproducible experimental results. It is presented in a fine powder form, which is easy to dissolve in water. The product is stored under standard conditions and has a relatively long shelf - life when properly preserved.
HEPES sodium salt has a wide range of applications in the scientific and medical fields. In cell culture, it is used as a buffering agent to maintain the physiological pH of the cell culture medium. This is crucial for the survival, growth, and normal functioning of cells in vitro. By keeping the pH stable, it helps to prevent cell damage and maintain the integrity of the cell membrane.
In biochemical assays, such as enzyme - catalyzed reactions, HEPES sodium salt provides a stable pH environment. Enzymes are highly sensitive to pH changes, and a stable pH is necessary for them to exhibit optimal activity. Therefore, using HEPES sodium salt can improve the accuracy and reliability of biochemical assays.
In molecular biology, it is also used in DNA and RNA extraction procedures. It helps to maintain the stability of nucleic acids during the extraction process and prevents their degradation.
When using HEPES sodium salt, first, calculate the appropriate amount according to the desired buffer concentration and volume. Dissolve the required amount of HEPES sodium salt in distilled or deionized water. Stir gently until it is completely dissolved. The solution can be adjusted to the desired pH value using a suitable acid or base, such as hydrochloric acid or sodium hydroxide.
For cell culture applications, prepare the culture medium according to the standard protocol and add the HEPES sodium salt buffer solution. Make sure the final concentration of the buffer in the medium meets the requirements of the cells being cultured. Filter - sterilize the medium before use to prevent contamination.
In biochemical assays, add the appropriate amount of HEPES sodium salt buffer to the reaction system at the beginning of the experiment. Ensure thorough mixing to achieve a homogeneous pH environment throughout the system.
A research team was studying the proliferation of human embryonic kidney (HEK) cells. They used a culture medium buffered with our HEPES sodium salt. Over a period of 7 days, the cells showed a significant increase in cell density and maintained normal morphology. In contrast, when using a medium with a less - effective buffer, the cell growth was stunted, and many cells exhibited signs of apoptosis. This clearly demonstrates the superiority of our HEPES sodium salt in maintaining a stable pH for cell culture.
In a biochemical assay for measuring the activity of a particular enzyme, the use of HEPES sodium salt buffer resulted in highly reproducible results. The enzyme activity was measured under different substrate concentrations, and the data showed a clear Michaelis - Menten kinetic curve. When a different buffer was used, the results were less consistent, with large variations in enzyme activity measurements, indicating the importance of a stable pH provided by HEPES sodium salt.
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