High Quality Tricine CAS 5704-04-1 is a premium chemical product offered at a low price. Its Chemical Abstracts Service (CAS) number is 5704-04-1. Tricine, also known as N-tris(Hydroxymethyl)methylglycine, is a zwitterionic amino acid buffer. It has a molecular formula of C6H13NO5 and a molecular weight of approximately 179.17 g/mol. The product features high purity, ensuring reliable performance in various applications. It typically comes in a white crystalline powder form, which is easy to handle and dissolve in aqueous solutions.
This high - quality Tricine has a wide range of applications. In biochemical research, it is commonly used as a buffer in electrophoresis systems. It helps maintain a stable pH environment, which is crucial for the separation and analysis of proteins and nucleic acids. Tricine is particularly suitable for the separation of low - molecular - weight proteins due to its unique buffering capacity and low conductivity. In cell culture, Tricine can be used to adjust the pH of the culture medium, providing a more stable and favorable growth environment for cells. It is also used in the formulation of some enzyme assays, where precise pH control is necessary for the proper functioning of enzymes.
When using Tricine, first, ensure that you are working in a clean and well - ventilated environment. Wear appropriate personal protective equipment, such as gloves and goggles. To prepare a Tricine buffer solution, weigh the appropriate amount of Tricine powder according to the desired concentration and volume. For example, to prepare a 0.1 M Tricine buffer solution, dissolve 17.917 g of Tricine in approximately 800 mL of deionized water. Then, adjust the pH of the solution to the desired value using an appropriate acid or base, such as hydrochloric acid or sodium hydroxide. After the pH adjustment, bring the total volume of the solution to 1 L with deionized water. Mix the solution thoroughly to ensure complete dissolution of the Tricine. Store the prepared buffer solution at an appropriate temperature, usually at 4°C to maintain its stability.
Case Study 1: Protein Electrophoresis
A research team was working on the separation of a group of low - molecular - weight proteins. They initially used a traditional buffer system but found that the separation was not satisfactory. After switching to a Tricine - based buffer system, the resolution of the protein bands improved significantly. The Tricine buffer maintained a stable pH during the electrophoresis process, allowing for clear separation of the proteins. This enabled the researchers to accurately identify and analyze the target proteins, which was crucial for their research on the protein functions.
Case Study 2: Cell Culture
A biotech company was culturing a particular type of mammalian cells. The cells were showing poor growth in the original culture medium. By adding Tricine to the medium to adjust the pH, the company noticed a significant improvement in cell growth. The Tricine - supplemented medium maintained a more stable pH, providing a better environment for cell proliferation. As a result, the company was able to increase the cell yield and improve the quality of the cells for further downstream applications, such as protein production.
If you have any questions or need further information about our High Quality Tricine CAS 5704 - 04 - 1, please feel free to contact us. Our email address is info@vivalr.com, and our phone number is (86) 15866781826. We are committed to providing you with the best products and services.
Este es William, CEO de Zhishang Chemical Co., Ltd.
Le invitamos a visitar nuestra fábrica. Como el fabricante de productos químicos superior, nos hemos estado esforzando para la calidad del producto, la innovación, el R&D, y el servicio de atención al cliente por los últimos 7 años. En los próximos 10 años o incluso más, nos comprometemos a convertirnos en el proveedor de productos químicos más fiable del mundo, creando una marca internacional conocida en la que confíen los clientes, con el espíritu de "un metro de ancho, diez mil metros de profundidad", seguimos centrándonos en la investigación y el desarrollo de productos, seguimos centrándonos en el servicio al cliente, seguimos mejorando el sistema de servicio de la cadena de suministro, para crear un equipo profesional de servicio de suministro de productos químicos, para lograr una cooperación a largo plazo en la que todos ganen. No dude en ponerse en contacto con nosotros si tiene alguna pregunta.
Spanish 
English (United States) 
English (Australia) 
Portuguese