The product is High Quality heparin lithium salt with the CAS number 9045 - 22 - 1. Heparin lithium salt is a derivative of heparin, a glycosaminoglycan widely used in the medical and biological fields. As a high - quality product, it meets strict quality standards and is offered at a competitive price.
Chemically, heparin lithium salt is a polysaccharide composed of repeating disaccharide units. It has a high negative charge density due to the presence of sulfate and carboxyl groups. The lithium salt form offers certain advantages in terms of solubility and compatibility in some applications compared to other salts of heparin.
The product is usually presented in a fine powder form, with a high degree of purity. It is stable under normal storage conditions, but should be kept away from excessive heat, moisture, and light to maintain its quality.
Medical Field: In the medical industry, heparin lithium salt is mainly used as an anticoagulant. It can prevent the formation of blood clots by inhibiting the activity of thrombin and other clotting factors. It is commonly used in blood collection tubes for laboratory tests. The lithium salt form is preferred in some cases because it does not interfere with certain biochemical assays as much as other anticoagulants.
Biological Research: In biological research, heparin lithium salt is used in cell culture and protein purification. It can bind to certain proteins and growth factors, protecting them from degradation and facilitating their isolation and purification. It is also used in studies related to cell - cell and cell - matrix interactions.
Blood Collection: When used in blood collection tubes, the appropriate amount of heparin lithium salt is pre - added to the tube. The recommended concentration is usually between 10 - 20 IU/mL of blood. After blood is drawn into the tube, gently invert the tube several times to ensure thorough mixing of the blood with the anticoagulant.
Cell Culture: In cell culture applications, heparin lithium salt can be added to the culture medium at a concentration of 1 - 10 μg/mL. It should be filtered through a 0.22 - μm filter before use to ensure sterility.
Protein Purification: For protein purification, heparin lithium salt can be used in affinity chromatography columns. The column is first equilibrated with a buffer containing heparin lithium salt, and then the protein sample is loaded. The target proteins that bind to heparin can be eluted using a gradient of salt concentration.
Case 1: Blood Testing in a Clinical Laboratory
A clinical laboratory was experiencing inaccurate results in certain biochemical assays due to the use of traditional anticoagulants. After switching to heparin lithium salt in blood collection tubes, the interference with the assays was significantly reduced. The laboratory tested over 1000 blood samples, and the coefficient of variation for key biochemical parameters such as glucose, cholesterol, and electrolytes decreased by an average of 15%. This improvement led to more reliable test results and better patient diagnosis.
Case 2: Protein Purification in a Research Lab
A research lab was trying to purify a growth factor from a cell culture supernatant. They used a heparin - based affinity chromatography column with heparin lithium salt. By optimizing the binding and elution conditions, they were able to purify the growth factor with a purity of over 95%. The purified growth factor was then used in cell proliferation assays, and it showed a significant increase in cell growth rate compared to the non - purified sample.
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