4-morpholineethanesulfonic acid, with the CAS number 4432-31-9, is a well - known buffer substance in the scientific community. The product we offer is factory - supplied with the best price, ensuring high - quality at a competitive cost.
Fórmula química: C₆H₁₃NO₄S
Peso molecular: Approximately 195.23 g/mol
Apariencia: It usually appears as a white crystalline powder. This form makes it easy to handle and store.
Solubilidad: It is highly soluble in water, which is a crucial property for its use in various aqueous - based applications.
pH Range: It has an effective buffering range of pH 5.5 - 7.7, which makes it suitable for a wide variety of biological and chemical reactions.
Biological Research: In cell culture, 4 - morpholineethanesulfonic acid is widely used as a buffer to maintain the pH stability of the culture medium. Cells are very sensitive to pH changes, and this buffer helps to create an optimal environment for cell growth, proliferation, and metabolism. In enzyme assays, it can also be used to ensure that the reaction occurs under the appropriate pH conditions, thus maintaining the activity of enzymes.
Biochemical Analysis: In electrophoresis, it serves as a buffer component. During the separation of biomolecules such as proteins and nucleic acids, a stable pH environment is necessary for accurate separation results. The use of 4 - morpholineethanesulfonic acid can help achieve this goal.
Industria farmacéutica: It can be used in the formulation of some drugs. As a buffer, it helps to maintain the stability of drug solutions, preventing the degradation of drugs due to pH changes and ensuring the efficacy and safety of drugs.
Buffer Preparation: To prepare a buffer solution, accurately weigh the appropriate amount of 4 - morpholineethanesulfonic acid according to the desired concentration and pH value. Dissolve it in distilled water, and then adjust the pH with a suitable acid or base, such as hydrochloric acid or sodium hydroxide. Stir well to ensure complete dissolution.
Almacenamiento: Store the product in a cool, dry place, away from light. The container should be tightly sealed to prevent moisture absorption and contamination. It is recommended to use the product within the expiration date to ensure its quality.
Cell Culture Case: A research laboratory was conducting a study on the growth of mammalian cells. They used a culture medium without proper buffering at first, and the cell growth was slow and inconsistent. After switching to a culture medium buffered with 4 - morpholineethanesulfonic acid at a concentration of 20 mM and a pH of 6.8, the cell growth rate significantly increased. The cells showed a more uniform morphology, and the cell viability also improved. This indicated that the stable pH environment provided by the buffer was beneficial for cell growth.
Enzyme Assay Case: In an enzyme - catalyzed reaction study, the researchers found that the enzyme activity was unstable when the reaction was carried out in a non - buffered solution. When they added 4 - morpholineethanesulfonic acid to the reaction system to maintain a pH of 7.2, the enzyme activity became stable, and the reaction rate was more predictable. This demonstrated the importance of using this buffer in enzyme - related research.
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