Factory Supply Fluorescein Sodium Salt, with the CAS number 518 - 47 - 8, is a well - known chemical compound. Chemically, it is the disodium salt of fluorescein. Its molecular formula is C20H10Na2O5, and it has a molecular weight of approximately 376.26 g/mol. This product usually appears as an orange - red powder that is highly soluble in water, producing a bright green - fluorescent solution.
The product is supplied by a factory, which means it can be obtained in bulk at a relatively good price. It is of high purity, meeting the industrial and scientific requirements, and has been carefully manufactured under strict quality control processes to ensure its stability and reliability.
1. **Medical Field**: In ophthalmology, Fluorescein Sodium Salt is widely used for diagnostic purposes. It can be injected into the bloodstream to visualize the blood vessels in the retina during a fluorescein angiography. This helps doctors detect various eye diseases such as diabetic retinopathy, macular degeneration, and blocked blood vessels in the eye.
2. **Biological Research**: It serves as a fluorescent tracer in cell biology and microbiology. Scientists can label cells or biomolecules with fluorescein sodium salt to track their movement, interactions, and distribution within a biological system. This is crucial for understanding biological processes at the cellular and molecular levels.
3. **Environmental Monitoring**: It can be used as a tracer in water systems. By adding a small amount of fluorescein sodium salt to water, researchers can study water flow patterns, detect leaks in pipes, and analyze the mixing of different water bodies.
1. **Medical Use**: In a medical setting, the fluorescein sodium salt is typically prepared as a sterile solution. The dosage is determined by the patient's condition and the type of examination. It is usually injected intravenously at a slow rate. After injection, the patient needs to wait for a short period for the dye to circulate in the body before the examination can be carried out. Special care should be taken to avoid extravasation during injection, as it may cause local tissue damage.
2. **Biological Research**: For biological research, the product is first dissolved in an appropriate buffer solution. The concentration of the solution depends on the specific experiment. Cells or biomolecules are then incubated with the fluorescein - containing solution for a certain period. After incubation, the samples are washed thoroughly to remove any unbound dye, and then they can be observed under a fluorescence microscope.
3. **Environmental Monitoring**: In water systems, a known amount of fluorescein sodium salt is added to the water source. Samples are then collected at different points and times to measure the fluorescence intensity. The data obtained can be used to calculate the flow rate, dispersion, and other parameters of the water system.
1. **Ophthalmology Case**: A 55 - year - old diabetic patient was experiencing vision problems. The doctor suspected diabetic retinopathy. A fluorescein angiography was performed using Fluorescein Sodium Salt. After injecting the dye, the doctor was able to clearly visualize the abnormal blood vessels in the patient's retina. This allowed for an accurate diagnosis and the development of an appropriate treatment plan, which significantly improved the patient's prognosis.
2. **Biological Research Case**: In a cell migration study, scientists used fluorescein - labeled cells. They added a small amount of fluorescein sodium salt to the cell culture medium. After incubation, the cells were labeled with fluorescence. The researchers then observed the migration of the cells under a microscope. By analyzing the changes in the fluorescence pattern over time, they were able to understand the mechanism of cell migration and identified potential targets for drug development.
3. **Environmental Monitoring Case**: A water treatment plant suspected a leak in its underground pipes. Fluorescein sodium salt was added to the water in the distribution system. Samples were collected from different points in the system. The presence of fluorescence in the soil near a particular area indicated a leak. This enabled the maintenance team to quickly locate and repair the leak, minimizing water loss.
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Este es William, CEO de Zhishang Chemical Co., Ltd.
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