{"id":2703,"date":"2025-03-07T12:07:44","date_gmt":"2025-03-07T12:07:44","guid":{"rendered":"https:\/\/vivalr.com\/product\/2703.html"},"modified":"2025-03-07T12:26:37","modified_gmt":"2025-03-07T12:26:37","slug":"high-quality-2-nitrophenyl-beta-d-galactopyranoside-cas-369-07-3-with-best-price","status":"publish","type":"product","link":"https:\/\/vivalr.com\/es\/product\/2703.html","title":{"rendered":"High Quality 2-Nitrophenyl-beta-D-galactopyranoside CAS 369-07-3 With Best Price"},"content":{"rendered":"<h1>User Guide for High Quality 2-Nitrophenyl-beta-D-galactopyranoside CAS 369-07-3 With Best Price<\/h1>\n<h2>\u00cdndice<\/h2>\n<ul>\n<li><a href=\"#basic_info\">1. Informaci\u00f3n b\u00e1sica y par\u00e1metros<\/a><\/li>\n<li><a href=\"#uses\">2. Usos del producto<\/a><\/li>\n<li><a href=\"#usage\">3. C\u00f3mo utilizar el producto<\/a><\/li>\n<li><a href=\"#case_studies\">4. Casos pr\u00e1cticos detallados<\/a><\/li>\n<li><a href=\"#contact\">5. 5. Informaci\u00f3n de contacto<\/a><\/li>\n<\/ul>\n<h2 id=\"basic_info\">1. Informaci\u00f3n b\u00e1sica y par\u00e1metros<\/h2>\n<p>The product named \"High Quality 2-Nitrophenyl-beta-D-galactopyranoside CAS 369-07-3 With Best Price\" is a well - known chemical compound. The CAS number, 369 - 07 - 3, is a unique identifier for this specific chemical, which helps in accurate identification and sourcing in the chemical market.<\/p>\n<p>2 - Nitrophenyl - beta - D - galactopyranoside is a synthetic substrate commonly used in biochemical research. It has a molecular formula that can be determined through advanced analytical techniques. Physically, it may present as a white or off - white powder, with a certain solubility in appropriate solvents such as water and some organic solvents. The purity of this high - quality product is strictly controlled during the manufacturing process to ensure reliable experimental results.<\/p>\n<h2 id=\"uses\">2. Usos del producto<\/h2>\n<p>2 - Nitrophenyl - beta - D - galactopyranoside is primarily used in the field of biochemistry and molecular biology. It is a substrate for the enzyme beta - galactosidase. When beta - galactosidase acts on 2 - Nitrophenyl - beta - D - galactopyranoside, it cleaves the galactopyranoside bond, releasing 2 - nitrophenol. The amount of 2 - nitrophenol released can be measured spectrophotometrically at a specific wavelength (usually around 420 nm), allowing for the quantification of beta - galactosidase activity.<\/p>\n<p>This property makes it widely used in enzyme assays. For example, in genetic research, it can be used to detect the expression of genes that encode beta - galactosidase. In the study of gene regulation, the change of beta - galactosidase activity can reflect the state of gene expression, providing important information for understanding gene function and regulatory mechanisms.<\/p>\n<h2 id=\"usage\">3. C\u00f3mo utilizar el producto<\/h2>\n<p>When using 2 - Nitrophenyl - beta - D - galactopyranoside, it is essential to follow good laboratory practices. First, prepare an appropriate buffer solution according to the experimental requirements. The buffer should have a suitable pH value to maintain the optimal activity of beta - galactosidase.<\/p>\n<p>Dissolve 2 - Nitrophenyl - beta - D - galactopyranoside in the buffer to prepare a substrate solution of a certain concentration. Add the enzyme sample to the substrate solution, and start the reaction. Incubate the reaction mixture at a specific temperature (usually around 37\u00b0C) for a certain period of time, depending on the specific experimental design.<\/p>\n<p>After the reaction is completed, stop the reaction by adding a stop solution. Then, measure the absorbance of the reaction mixture at the appropriate wavelength using a spectrophotometer. Based on the standard curve, the concentration of the product (2 - nitrophenol) can be calculated, and then the activity of beta - galactosidase can be determined.<\/p>\n<h2 id=\"case_studies\">4. Casos pr\u00e1cticos detallados<\/h2>\n<p>Case Study 1: Gene Expression Detection in Bacteria<br \/>\n        In a study of E. coli gene expression, researchers used 2 - Nitrophenyl - beta - D - galactopyranoside to detect the expression of the lacZ gene, which encodes beta - galactosidase. They first cultured E. coli under different growth conditions, including the presence and absence of lactose. Then, they prepared cell lysates and added them to the substrate solution containing 2 - Nitrophenyl - beta - D - galactopyranoside.<br \/>\n        After a 30 - minute incubation at 37\u00b0C, they stopped the reaction and measured the absorbance. The results showed that in the presence of lactose, the expression of the lacZ gene increased significantly, as indicated by a higher absorbance value, which was consistent with the theory of lac operon regulation.<\/p>\n<p>Case Study 2: Enzyme Inhibition Study<br \/>\n        A group of researchers was interested in studying the inhibitors of beta - galactosidase. They prepared different concentrations of an inhibitor and added them to the reaction mixture containing 2 - Nitrophenyl - beta - D - galactopyranoside and beta - galactosidase. By measuring the absorbance at different time points, they found that the inhibitor could effectively reduce the activity of beta - galactosidase in a dose - dependent manner, which provided valuable information for drug development targeting this enzyme.<\/p>\n<h2 id","protected":false},"excerpt":{"rendered":"<p>User Guide for High Quality 2-Nitrophenyl-beta-D-galactopyranoside CAS 369-07-3 With Best Price Table of Contents 1. Basic Information and Parameters 2. Uses of the Product 3. How to Use the Product 4. Detailed Usage Case Studies 5. Contact Information 1. Basic Information and Parameters The product named \"High Quality 2-Nitrophenyl-beta-D-galactopyranoside CAS 369-07-3 With Best Price\" is [&hellip;]<\/p>\n","protected":false},"featured_media":22503,"comment_status":"closed","ping_status":"open","template":"","meta":[],"product_brand":[],"product_cat":[15],"product_tag":[],"class_list":{"0":"post-2703","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-uncategorized","8":"first","9":"instock","10":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v24.4 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>High Quality 2-Nitrophenyl-beta-D-galactopyranoside CAS 369-07-3 With Best Price - Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/vivalr.com\/es\/product\/2703.html\" \/>\n<meta property=\"og:locale\" content=\"es_MX\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"High Quality 2-Nitrophenyl-beta-D-galactopyranoside CAS 369-07-3 With Best Price\" \/>\n<meta property=\"og:description\" content=\"User Guide for High Quality 2-Nitrophenyl-beta-D-galactopyranoside CAS 369-07-3 With Best Price Table of Contents 1. 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