{"id":913,"date":"2025-03-07T12:05:57","date_gmt":"2025-03-07T12:05:57","guid":{"rendered":"https:\/\/vivalr.com\/product\/913.html"},"modified":"2025-03-07T12:23:37","modified_gmt":"2025-03-07T12:23:37","slug":"factory-supply-33%e2%80%b2-diaminobenzidine-tetrahydrochloride-hydrate-cas-868272-85-9-with-good-price","status":"publish","type":"product","link":"https:\/\/vivalr.com\/es\/product\/913.html","title":{"rendered":"Factory Supply 3,3\u2032-Diaminobenzidine tetrahydrochloride hydrate CAS 868272-85-9 With Good Price"},"content":{"rendered":"<div class=\"user-guide\">\n<h1>3,3\u2032-Diaminobenzidine Tetrahydrochloride Hydrate (CAS 868272-85-9) User Guide<\/h1>\n<div class=\"toc\">\n<h2>\u00cdndice<\/h2>\n<ul>\n<li><a href=\"#section1\">1. Especificaciones del producto<\/a><\/li>\n<li><a href=\"#section2\">2. Aplicaciones<\/a><\/li>\n<li><a href=\"#section3\">3. Instrucciones de uso<\/a><\/li>\n<li><a href=\"#section4\">4. Casos de uso detallados<\/a><\/li>\n<\/ul><\/div>\n<div id=\"section1\" class=\"section\">\n<h2>1. Especificaciones del producto<\/h2>\n<p><strong>Nombre del producto:<\/strong> 3,3\u2032-Diaminobenzidine Tetrahydrochloride Hydrate<\/p>\n<p><strong>N\u00famero CAS:<\/strong> 868272-85-9<\/p>\n<p><strong>F\u00f3rmula molecular:<\/strong> C<sub>12<\/sub>H<sub>16<\/sub>Cl<sub>4<\/sub>N<sub>4<\/sub>-xH<sub>2<\/sub>O<\/p>\n<p><strong>Apariencia:<\/strong> Light brown to tan crystalline powder<\/p>\n<p><strong>Pureza:<\/strong> \u226598% (HPLC)<\/p>\n<p><strong>Almacenamiento:<\/strong> Store at 2-8\u00b0C in a dark, dry environment<\/p>\n<\/p><\/div>\n<div id=\"section2\" class=\"section\">\n<h2>2. Aplicaciones<\/h2>\n<p>3,3\u2032-Diaminobenzidine (DAB) Tetrahydrochloride Hydrate is widely used as a chromogen in:<\/p>\n<ul>\n<li>Immunohistochemistry (IHC) for peroxidase-based staining<\/li>\n<li>Western blotting to detect proteins<\/li>\n<li>ELISA assays for signal visualization<\/li>\n<li>Histological staining in research and diagnostics<\/li>\n<\/ul><\/div>\n<div id=\"section3\" class=\"section\">\n<h2>3. Instrucciones de uso<\/h2>\n<ol>\n<li><strong>Preparaci\u00f3n de la soluci\u00f3n:<\/strong> Dissolve 5-10 mg of DAB in 10 mL of buffer (e.g., Tris-HCl, pH 7.6).<\/li>\n<li><strong>Substrate Reaction:<\/strong> Add 0.01% hydrogen peroxide to activate the solution.<\/li>\n<li><strong>Staining:<\/strong> Apply the solution to samples and incubate for 1-10 minutes at room temperature.<\/li>\n<li><strong>Termination:<\/strong> Rinse thoroughly with distilled water to stop the reaction.<\/li>\n<li><strong>Seguridad:<\/strong> Wear gloves and eye protection; avoid inhalation or direct contact.<\/li>\n<\/ol><\/div>\n<div id=\"section4\" class=\"section\">\n<h2>4. Casos de uso detallados<\/h2>\n<h3>Case 1: Immunohistochemistry (IHC)<\/h3>\n<p>In IHC, DAB produces a brown precipitate when reacting with horseradish peroxidase (HRP)-conjugated antibodies. Steps:<\/p>\n<ol>\n<li>Deparaffinize and rehydrate tissue sections.<\/li>\n<li>Block endogenous peroxidase activity with 3% H<sub>2<\/sub>O<sub>2<\/sub>.<\/li>\n<li>Incubate with DAB solution for 5 minutes; monitor under a microscope.<\/li>\n<li>Counterstain with hematoxylin and mount slides.<\/li>\n<\/ol>\n<h3>Case 2: Western Blotting<\/h3>\n<p>For protein detection:<\/p>\n<ol>\n<li>Transfer proteins to a PVDF membrane.<\/li>\n<li>Block with 5% non-fat milk, then incubate with HRP-conjugated secondary antibody.<\/li>\n<li>Apply DAB substrate for 2-3 minutes; wash and air-dry.<\/li>\n<li>Analyze bands using imaging software.<\/li>\n<\/ol><\/div>\n<div class=\"contact\">\n<p><strong>P\u00f3ngase en contacto con nosotros:<\/strong><br \/>\n        Correo electr\u00f3nico: <a href=\"mailto:info@vivalr.com\">info@vivalr.com<\/a><br \/>\n        Tel\u00e9fono: (86) 15866781826<\/p>\n<\/p><\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>3,3\u2032-Diaminobenzidine Tetrahydrochloride Hydrate (CAS 868272-85-9) User Guide Table of Contents 1. Product Specifications 2. Applications 3. Usage Instructions 4. Detailed Use Cases 1. Product Specifications Product Name: 3,3\u2032-Diaminobenzidine Tetrahydrochloride Hydrate CAS Number: 868272-85-9 Molecular Formula: C12H16Cl4N4\u00b7xH2O Appearance: Light brown to tan crystalline powder Purity: \u226598% (HPLC) Storage: Store at 2-8\u00b0C in a dark, dry environment [&hellip;]<\/p>\n","protected":false},"featured_media":21616,"comment_status":"closed","ping_status":"open","template":"","meta":[],"product_brand":[],"product_cat":[15],"product_tag":[],"class_list":{"0":"post-913","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-uncategorized","8":"first","9":"instock","10":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v24.4 (Yoast SEO v28.1) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Factory Supply 3,3\u2032-Diaminobenzidine tetrahydrochloride hydrate CAS 868272-85-9 With Good Price - Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/vivalr.com\/es\/product\/913.html\" \/>\n<meta property=\"og:locale\" content=\"es_MX\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Factory Supply 3,3\u2032-Diaminobenzidine tetrahydrochloride hydrate CAS 868272-85-9 With Good Price\" \/>\n<meta property=\"og:description\" content=\"3,3\u2032-Diaminobenzidine Tetrahydrochloride Hydrate (CAS 868272-85-9) User Guide Table of Contents 1. 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Product Specifications Product Name: 3,3\u2032-Diaminobenzidine Tetrahydrochloride Hydrate CAS Number: 868272-85-9 Molecular Formula: C12H16Cl4N4\u00b7xH2O Appearance: Light brown to tan crystalline powder Purity: \u226598% (HPLC) Storage: Store at 2-8\u00b0C in a dark, dry environment [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/vivalr.com\/es\/product\/913.html\" \/>\n<meta property=\"og:site_name\" content=\"Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01\" \/>\n<meta property=\"article:modified_time\" content=\"2025-03-07T12:23:37+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/vivalr.com\/wp-content\/uploads\/2025\/03\/868272-85-9.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"350\" \/>\n\t<meta property=\"og:image:height\" content=\"350\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tiempo de lectura\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minuto\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/913.html\",\"url\":\"https:\\\/\\\/vivalr.com\\\/product\\\/913.html\",\"name\":\"Factory Supply 3,3\u2032-Diaminobenzidine tetrahydrochloride hydrate CAS 868272-85-9 With Good Price - Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/vivalr.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/913.html#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/913.html#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/vivalr.com\\\/wp-content\\\/uploads\\\/2025\\\/03\\\/868272-85-9.jpg\",\"datePublished\":\"2025-03-07T12:05:57+00:00\",\"dateModified\":\"2025-03-07T12:23:37+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/913.html#breadcrumb\"},\"inLanguage\":\"es\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/vivalr.com\\\/product\\\/913.html\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"es\",\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/913.html#primaryimage\",\"url\":\"https:\\\/\\\/vivalr.com\\\/wp-content\\\/uploads\\\/2025\\\/03\\\/868272-85-9.jpg\",\"contentUrl\":\"https:\\\/\\\/vivalr.com\\\/wp-content\\\/uploads\\\/2025\\\/03\\\/868272-85-9.jpg\",\"width\":350,\"height\":350},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/913.html#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/vivalr.com\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Shop\",\"item\":\"https:\\\/\\\/vivalr.com\\\/shop\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Factory Supply 3,3\u2032-Diaminobenzidine tetrahydrochloride hydrate CAS 868272-85-9 With Good Price\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/vivalr.com\\\/#website\",\"url\":\"https:\\\/\\\/vivalr.com\\\/\",\"name\":\"Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01\",\"description\":\"Discover a wide range of high-quality chemical raw materials to meet your industrial needs. 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Product Specifications 2. Applications 3. Usage Instructions 4. Detailed Use Cases 1. 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