Ethyl bromopyruvate (CAS No.: 70 - 23 - 5) is a high - quality chemical product. It is a colorless to light - yellow liquid with a molecular formula of C₅H₇BrO₃ and a molecular weight of approximately 195.01 g/mol. The product is known for its high purity and stable quality, which makes it a popular choice in various industries. It should be stored in a cool, dry place away from direct sunlight and heat sources to maintain its quality over time.
Ethyl bromopyruvate has a wide range of applications. In the field of organic synthesis, it serves as an important intermediate. It can be used in the preparation of various heterocyclic compounds, which are widely used in the pharmaceutical and agrochemical industries. For example, it can participate in substitution reactions and condensation reactions to form complex molecular structures. In the biomedical field, recent research has shown that ethyl bromopyruvate has potential anti - tumor properties. It can inhibit the growth of cancer cells by interfering with their energy metabolism pathways. Additionally, it can also be used in the development of new drugs targeting specific metabolic enzymes.
When using ethyl bromopyruvate, strict safety precautions should be taken. It is recommended to wear appropriate protective equipment, such as gloves, goggles, and a lab coat. Before use, ensure that the working environment is well - ventilated. When handling the product, use a pipette or other suitable devices to accurately measure the required amount. It is usually dissolved in organic solvents such as dichloromethane or ethanol for chemical reactions. The reaction conditions, such as temperature, reaction time, and the molar ratio of reactants, should be strictly controlled according to the specific reaction requirements. For example, in some substitution reactions, the reaction may need to be carried out under reflux conditions for several hours.
1. **Organic Synthesis Case**: A research group was synthesizing a new type of heterocyclic compound for potential pharmaceutical applications. They used ethyl bromopyruvate as a key intermediate. By reacting it with a specific amine compound in the presence of a base, they successfully obtained the target heterocyclic product. The reaction was carried out in dichloromethane at room temperature for 24 hours, followed by purification through column chromatography. The yield of the final product was about 70%, which was considered a good result in this type of synthesis.
2. **Biomedical Case**: In a cancer research project, scientists investigated the anti - tumor effect of ethyl bromopyruvate on a specific type of liver cancer cells. They treated the cancer cells with different concentrations of ethyl bromopyruvate in vitro. After 48 hours of treatment, they found that the growth of cancer cells was significantly inhibited at a relatively low concentration. Further studies showed that ethyl bromopyruvate disrupted the energy metabolism of cancer cells by inhibiting a key enzyme in the glycolytic pathway. This case demonstrated the potential of ethyl bromopyruvate as a novel anti - cancer agent.
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