1. Product Information

Methyl Desoxycholate (CAS 3245-38-3) is a high-purity biochemical reagent widely used in research and industrial applications. Key specifications include:

• Chemical Formula: C₂₅H₄₂O₄
• Molecular Weight: 406.6 g/mol
• Appearance: White crystalline powder
• Purity: ≥98% (HPLC)
• Solubility: Soluble in ethanol, methanol; slightly soluble in water
• Storage: Store at 2-8°C in a dry, dark environment

2. Product Uses

Methyl Desoxycholate is primarily employed in:

• Cell Lysis: Disrupts cell membranes for protein extraction in microbiology studies.
• Lipid Research: Acts as a detergent in lipid solubilization and liposome preparation.
• Diagnostic Reagents: Used in bile acid analysis and cholesterol metabolism assays.
• Pharmaceuticals: Intermediate in steroid synthesis and drug delivery systems.

3. Usage Guidelines

Preparation & Handling

• Dissolve in ethanol/methanol at 10-20 mg/mL for stock solutions.
• Use gloves and goggles; avoid inhalation or skin contact.
• Centrifuge solutions at 10,000×g for 5 mins to remove particulates.

Optimization Tips

• Adjust concentration (0.1%-1%) based on cell type and experimental goals.
• Combine with protease inhibitors during cell lysis to prevent protein degradation.

4. Case Studies

Case Study 1: Protein Extraction from E. coli

• Objective: Isolate cytoplasmic proteins.
• Method: 0.5% Methyl Desoxycholate in Tris buffer (pH 7.4), 30-min incubation at 4°C.
• Result: 95% cell lysis efficiency with minimal membrane protein contamination.

Case Study 2: Liposome Preparation for Drug Delivery

• Objective: Create stable doxorubicin-loaded liposomes.
• Method: Mixed with phosphatidylcholine (2:1 ratio) using thin-film hydration.
• Result: Achieved 85% drug encapsulation efficiency with 72-hour stability.

5. Contact Information

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