Table of Contents

1. Basic Information and Parameters
2. Usage of the Product
3. How to Use the Product
4. Detailed Case Studies
5. Contact Information

Basic Information and Parameters

The product is High Quality CAPS Sodium Salt, with the CAS number 105140 - 23 - 6. It is well - known for its high quality and is available at the best price in the market. The CAPS sodium salt is a zwitterionic buffer, which is commonly used in biological and biochemical research. It has a pH range of 9.7 - 11.1 and is often used to maintain the stability of the pH in various experimental systems. The chemical formula of CAPS sodium salt is C₉H₁₈NNaO₃S, and its molecular weight is approximately 243.29 g/mol.

Usage of the Product

CAPS sodium salt has a wide range of applications. In the field of biochemistry, it is frequently used in protein electrophoresis. It can provide a stable alkaline environment, which is crucial for the separation and analysis of proteins. Since many proteins have different charges and conformations at different pH values, using CAPS sodium salt as a buffer can help to accurately separate proteins according to their molecular weight and charge.

In addition, it is also used in enzyme activity assays. Enzymes are highly sensitive to the pH of the reaction environment. CAPS sodium salt can maintain the optimal pH for enzyme - catalyzed reactions, ensuring the accuracy and reproducibility of the assay results. Moreover, it is used in cell culture media. Some cell types require a specific alkaline pH for growth and survival, and CAPS sodium salt can be used to adjust and maintain the appropriate pH in the culture medium.

How to Use the Product

When using High Quality CAPS Sodium Salt, it is important to follow the proper procedures. First, calculate the amount of CAPS sodium salt needed according to the desired concentration and volume of the buffer solution. Usually, it can be dissolved in deionized water. Gently stir the solution to ensure complete dissolution. The solution may need to be filtered through a 0.22 - micron filter to remove any particulate matter.

When preparing a buffer for protein electrophoresis, add appropriate additives according to the specific experiment requirements, such as SDS (sodium dodecyl sulfate) for SDS - PAGE. Adjust the pH of the solution using an appropriate acid or base, and use a pH meter to accurately measure and adjust the pH within the desired range. For enzyme activity assays, add the substrate and enzyme to the buffer solution prepared with CAPS sodium salt and incubate at the appropriate temperature for the reaction to occur.

Detailed Case Studies

Case 1: Protein Separation in a Research Laboratory

A research team was studying a new type of membrane protein. They used CAPS sodium salt to prepare a buffer for SDS - PAGE. By precisely adjusting the pH using CAPS sodium salt buffer, they were able to achieve clear separation of the target protein from other impurities. The clear bands on the gel allowed them to further analyze the molecular weight and purity of the protein. This successful separation was crucial for their subsequent research on the protein's function and structure.

Case 2: Enzyme Activity Assay in a Pharmaceutical Company

A pharmaceutical company was developing a new drug that targeted a specific enzyme. To accurately measure the enzyme activity, they used CAPS sodium salt buffer in their assay system. The stable alkaline environment provided by the buffer ensured that the enzyme maintained its maximum activity. As a result, they were able to accurately evaluate the inhibitory effect of the new drug candidate on the enzyme, which was an important step in the drug development process.

Contact Information

If you have any questions about High Quality CAPS Sodium Salt, please feel free to contact us. Our email is info@vivalr.com, and our phone number is (86) 15866781826. We are happy to provide you with more detailed information and professional advice.