Objective: Measure β-galactosidase activity using 5 mM METHYL-β-D-GALACTOPYRANOSIDE as a substrate in pH 7.4 buffer. Monitor hydrolysis via spectrophotometry at 410 nm. Resulted in Km value of 2.3 mM, confirming substrate specificity.
Protocol: Pre-incubate 10 µM compound with enzyme and 5 mM substrate. Reduced hydrolysis rate by 65%, identifying a potent galactosidase inhibitor for therapeutic development.
Process: Used as a glycosyl donor in enzymatic synthesis of oligosaccharides. Achieved 82% yield of target trisaccharide via transglycosylation reaction.
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