The product is named High Quality Tryptone CAS 91079 - 83 - 3 With Best Price. Tryptone is a mixture of peptides derived from the digestion of casein by the pancreatic enzyme trypsin. The CAS number 91079 - 83 - 3 uniquely identifies this particular chemical compound in the scientific and commercial community.
High - quality tryptone typically has the following characteristics: It has a high nitrogen content, usually around 14 - 16%. The pH value of a 1% solution at 25°C is generally in the range of 6.8 - 7.2. It is a yellow - to - tan, free - flowing powder with a characteristic odor. The moisture content is relatively low, often less than 5%, which helps to maintain its stability during storage. It is soluble in water, forming a clear to slightly opalescent solution. This product is usually available in various packaging sizes, such as 250g, 500g, and 1kg, to meet different user needs.
High - quality tryptone has a wide range of applications in the fields of microbiology and biotechnology:
Microbial Culture Media: It is one of the most commonly used components in microbiological culture media. Tryptone provides a rich source of amino acids, peptides, and nitrogen for the growth of various microorganisms, including bacteria, fungi, and yeasts. For example, in the preparation of nutrient agar, tryptone is used to support the growth of a wide variety of heterotrophic bacteria. In selective media, it can be combined with other ingredients to selectively promote the growth of certain types of microorganisms while inhibiting others.
Biotechnology Research: In biotechnology research, tryptone is used in cell culture and fermentation processes. It can be used as a nutrient source in the production of recombinant proteins, antibiotics, and other biotechnological products. For example, in the fermentation of Escherichia coli to produce recombinant proteins, tryptone - containing media can significantly improve the growth rate and protein production efficiency of the bacteria.
Media Preparation: When preparing culture media, first calculate the required amount of tryptone according to the formula of the media. Then, add the appropriate amount of tryptone to distilled or deionized water. Stir the mixture well until the tryptone is completely dissolved. If necessary, adjust the pH of the solution using an appropriate acid or base. After that, add other components of the media, such as salts, sugars, and vitamins. Finally, autoclave the media at 121°C for 15 - 20 minutes to sterilize it.
Storage: Store tryptone in a cool, dry place away from direct sunlight. Keep the container tightly closed to prevent moisture and contamination. If stored properly, tryptone can maintain its quality for an extended period.
Case 1: Bacterial Isolation and Identification
A research team in a microbiology laboratory was trying to isolate and identify bacteria from a soil sample. They prepared a nutrient agar medium containing tryptone. After inoculating the soil sample on the agar plates and incubating them at 37°C for 24 hours, a large number of bacterial colonies grew on the plates. By observing the morphological characteristics of the colonies and performing biochemical tests, they were able to identify several different types of bacteria, including Bacillus subtilis and Escherichia coli. This case shows that tryptone - containing media can effectively support the growth of bacteria from environmental samples, facilitating bacterial isolation and identification.
Case 2: Recombinant Protein Production
A biotechnology company was engaged in the production of a recombinant therapeutic protein using Escherichia coli as the host. They optimized the fermentation medium by using high - quality tryptone. By adjusting the concentration of tryptone and other components in the medium, they were able to increase the cell density and protein production of E. coli significantly. The yield of the recombinant protein increased from 50 mg/L to 150 mg/L, which greatly improved the production efficiency and economic benefits of the company.
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