Factory Supply TAPS CAS 29915-38-6 With Low Price: Comprehensive User Guide
Índice
1. Informações sobre o produto
Nome do produto: TAPS (CAS 29915-38-6)
Nome químico: Tris(hydroxymethyl)methylaminopropanesulfonic acid
Fórmula molecular: C7H17NO6S
Peso molecular: 243.28 g/mol
Aparência: Pó cristalino branco
Pureza: ≥99%
Armazenamento: Room temperature, dry environment
2. Applications of TAPS
TAPS (CAS 29915-38-6) is a zwitterionic biological buffer widely used in:
- Biochemical research and molecular biology experiments
- Cell culture media preparation
- Diagnostic reagent formulations
- Fabrico de produtos farmacêuticos
Its effective buffering range is pH 7.7–9.1, making it ideal for stabilizing pH in sensitive biological systems. TAPS also exhibits low binding affinity to metal ions, ensuring minimal interference in enzymatic reactions.
3. Diretrizes de utilização
Etapas de preparação:
- Dissolve TAPS in distilled water at a concentration of 0.1–1.0 M.
- Adjust pH using NaOH or HCl as required.
- Filter-sterilize the solution (0.22 µm filter) for cell culture applications.
Precauções:
- Store in a tightly sealed container to avoid moisture absorption.
- Wear protective gloves and goggles during handling.
- Test compatibility with other compounds before large-scale use.
4. Casos de utilização pormenorizados
Case 1: Cell Culture Media Preparation
Passo 1: Dissolve 22.33 g of TAPS in 800 mL distilled water.
Passo 2: Adjust pH to 8.5 with 1M NaOH.
Passo 3: Sterilize by filtration and add to DMEM medium at 25 mM final concentration.
Resultado: Stable pH maintenance during mammalian cell growth.
Case 2: Diagnostic Reagent Formulation
Passo 1: Prepare 0.5M TAPS solution (pH 9.0).
Passo 2: Mix with enzyme substrates and stabilizers.
Resultado: Enhanced accuracy in alkaline phosphatase-based assays.
5. Informações de contacto
Para encomendas em massa ou assistência técnica:
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- Telefone: (86) 15866781826