Factory Supply Chymotrypsinogen A with CAS number 9035 - 75 - 0 is a high - quality product offered at a low price. Chymotrypsinogen A is a zymogen (inactive precursor) of chymotrypsin, an enzyme that plays a vital role in the digestive system. It is a protein in nature, consisting of a specific amino - acid sequence.
The purity of our factory - supplied Chymotrypsinogen A is carefully controlled to ensure high - performance outcomes. Usually, it has a purity level of over [X]%, which can be further customized according to specific customer requirements. In terms of appearance, it typically presents as a white to off - white powder. The molecular weight of Chymotrypsinogen A is [X] kDa, and its isoelectric point is around [X]. These parameters are essential for understanding the physical and chemical properties of the product.
Chymotrypsinogen A has a wide range of applications in various fields:
When using Factory Supply Chymotrypsinogen A, the following steps are recommended:
Reconstitution: First, reconstitute the lyophilized powder in an appropriate buffer solution. The choice of buffer depends on the specific application. For most in - vitro experiments, a phosphate - buffered saline (PBS) with a pH of around 7.4 is commonly used. The reconstitution concentration should be determined according to the experimental or production requirements. Generally, a concentration of [X] mg/mL is a good starting point.
Armazenamento: After reconstitution, if not used immediately, the solution should be stored at - 20°C or lower to maintain its activity. Avoid repeated freeze - thaw cycles, as they can significantly reduce the enzyme's activity.
Usage in Experiments: When using it in experiments, add the appropriate volume of the reconstituted solution to the reaction system. The reaction conditions such as temperature, pH, and incubation time should be optimized according to the specific experimental design. For example, for proteolysis experiments, the reaction is usually carried out at 37°C for [X] hours.
Case 1: Proteomics Research
A research team at a well - known university was studying a group of novel proteins in a particular cell line. They used our Factory Supply Chymotrypsinogen A to cleave these proteins into peptides. First, they reconstituted the Chymotrypsinogen A in a Tris - HCl buffer (pH 8.0) at a concentration of 1 mg/mL. Then, they added the enzyme solution to the protein samples at an enzyme - to - substrate ratio of 1:50. After incubating the mixture at 37°C for 4 hours, they analyzed the resulting peptides using mass spectrometry. The high - purity Chymotrypsinogen A enabled them to obtain a clear and comprehensive peptide map, which greatly facilitated the identification and characterization of the novel proteins.
Caso 2: Produção farmacêutica
A pharmaceutical company was developing a digestive enzyme preparation. They used our Chymotrypsinogen A as one of the key ingredients. They optimized the formulation by adjusting the concentration of Chymotrypsinogen A and other enzymes
Este é o William, Diretor Executivo da Zhishang Chemical Co., Ltd.
Bem-vindo a visitar a nossa fábrica. Como o principal fabricante de produtos químicos, temos nos esforçado para a qualidade do produto, inovação, P & D e atendimento ao cliente nos últimos 7 anos. Nos próximos 10 anos ou mais, estamos empenhados em nos tornarmos o fornecedor de produtos químicos mais confiável do mundo, criando uma marca internacional bem conhecida e de confiança dos clientes, com o espírito de "um metro de largura, dez mil metros de profundidade", continuamos a nos concentrar na pesquisa e desenvolvimento de produtos, continuamos a nos concentrar no atendimento ao cliente, continuamos a melhorar o sistema de serviço da cadeia de suprimentos, para criar uma equipe profissional de serviços de fornecimento de produtos químicos, para alcançar uma cooperação de longo prazo ganha-ganha. Por favor, não hesite em contactar-nos se tiver alguma dúvida.
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