The product, High Quality N-(3-Sulfopropyl)-3,3′,5,5′-tetramethylbenzidine sodium salt with CAS number 102062-36-2, is a high - quality chemical compound. It is often used in various biochemical and medical research fields. The sodium salt form provides enhanced solubility and stability in aqueous solutions, which is beneficial for many applications. The compound has a specific molecular structure that gives it unique chemical and physical properties.
Regarding its chemical formula, it is designed to meet high - purity standards, usually with a purity level of over 98%. This high purity ensures reliable and reproducible results in experiments and applications. The compound is typically supplied in a fine - powder form, which allows for easy weighing and dissolution.
This product has a wide range of applications. In the field of enzyme - linked immunosorbent assay (ELISA), it serves as a chromogenic substrate. When an enzyme in the ELISA system reacts with this compound, a color change occurs, which can be measured spectrophotometrically. This color change is proportional to the amount of the target analyte in the sample, making it a crucial component for quantitative analysis in ELISA.
It is also used in immunohistochemistry. In this application, it helps to visualize the presence and distribution of specific proteins or antigens in tissue samples. By reacting with the enzyme - labeled antibodies, it produces a visible signal, enabling researchers to study the localization and expression levels of target molecules.
Before using the product, it is important to wear appropriate personal protective equipment, such as gloves and goggles, to avoid direct contact. When preparing a solution, dissolve the powder in an appropriate buffer. The recommended buffer is usually a phosphate - buffered saline (PBS) solution with a pH around 7.2 - 7.4.
For ELISA, add the substrate solution to the wells of the microplate after the appropriate washing steps. Incubate the plate at room temperature for a specific period, usually 15 - 30 minutes, depending on the protocol. The reaction can be stopped by adding a stop solution, and then the absorbance can be measured at a specific wavelength, typically 450 nm.
In immunohistochemistry, apply the substrate solution to the tissue sections after the antibody - incubation steps. Incubate for an appropriate time, and then rinse the sections to remove the unreacted substrate. Finally, the stained sections can be observed under a microscope.
Case 1: A research team was studying the expression level of a particular biomarker in cancer patients. They used this N-(3 - Sulfopropyl)-3,3′,5,5′ - tetramethylbenzidine sodium salt in an ELISA assay. By measuring the absorbance values, they were able to accurately quantify the biomarker concentration in patient serum samples. This data provided important insights into the progression of the disease and helped in evaluating the effectiveness of a new treatment.
Case 2: In a histology laboratory, researchers were interested in studying the distribution of a specific protein in mouse brain tissue. They employed immunohistochemistry with this compound as the chromogenic substrate. After staining the tissue sections, they were able to clearly visualize the localization of the protein in different brain regions. This information was valuable for understanding the neural function and possible pathological mechanisms.
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Este é o William, Diretor Executivo da Zhishang Chemical Co., Ltd.
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