The product, High Quality Disodium 4 - Nitrophenylphosphate CAS 4264 - 83 - 9 With Best Price, is a high - quality chemical compound. Its CAS number, 4264 - 83 - 9, serves as a unique identifier in the chemical industry. The molecular formula of disodium 4 - nitrophenylphosphate is C6H4NÃO6PNa2, and its molecular weight is approximately 263.05 g/mol. This product is usually in the form of a white to off - white powder. It is highly soluble in water, which makes it convenient for various applications.
Disodium 4 - nitrophenylphosphate has a wide range of applications. In the field of biochemistry, it is commonly used as a substrate for phosphatases. Phosphatases are enzymes that catalyze the hydrolysis of phosphate esters. When disodium 4 - nitrophenylphosphate is hydrolyzed by phosphatases, it produces 4 - nitrophenol, which has a yellow color. This color change can be measured spectrophotometrically, allowing for the quantification of phosphatase activity. In addition, it is also used in the development of diagnostic kits for detecting diseases related to abnormal phosphatase levels. For example, in the diagnosis of liver and bone diseases, the measurement of alkaline phosphatase activity using disodium 4 - nitrophenylphosphate can provide valuable information for doctors.
When using disodium 4 - nitrophenylphosphate, first, it is necessary to prepare an appropriate buffer solution. The buffer solution should have a suitable pH value, usually around 7 - 8, depending on the specific requirements of the experiment. Then, accurately weigh the required amount of disodium 4 - nitrophenylphosphate and dissolve it in the buffer solution. The concentration of the solution can be adjusted according to the experimental needs. For enzyme activity assays, a typical concentration may range from 1 - 10 mM. After preparing the substrate solution, add it to the reaction system containing the enzyme. Incubate the reaction mixture at an appropriate temperature, usually 37°C, for a certain period of time, typically 10 - 30 minutes. Finally, measure the absorbance of the reaction solution at a specific wavelength (usually 405 nm for 4 - nitrophenol) to determine the enzyme activity.
In a research project aiming to study the effect of a new drug on the activity of alkaline phosphatase in liver cells, disodium 4 - nitrophenylphosphate was used as a substrate. The researchers first cultured liver cells in vitro and divided them into two groups: a control group and a treatment group. The treatment group was treated with the new drug, while the control group was not. Then, the cells were lysed, and the lysates were used for the alkaline phosphatase activity assay. The substrate solution of disodium 4 - nitrophenylphosphate was prepared at a concentration of 5 mM in a Tris - HCl buffer (pH 7.5). An appropriate amount of the cell lysate was added to the substrate solution and incubated at 37°C for 20 minutes. After the incubation, the absorbance at 405 nm was measured using a spectrophotometer. The results showed that the alkaline phosphatase activity in the treatment group was significantly lower than that in the control group, indicating that the new drug had an inhibitory effect on alkaline phosphatase in liver cells.
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