High Quality Ribonuclease A with CAS number 9001 - 99 - 4 is a well - known enzyme. Ribonuclease A is a pancreatic ribonuclease that specifically digests single - stranded RNA at the 3' end of pyrimidine nucleotides, cleaving the phosphodiester bonds. The product is of high quality, which means it has high purity and activity.
The enzyme is typically available in a lyophilized powder form for better storage stability. It has a molecular weight of approximately 13.7 kDa. The optimal pH range for its activity is around 7 - 8, and the optimal temperature is usually 37°C. This enzyme is highly specific for RNA substrates and has little to no activity against DNA.
Ribonuclease A has a wide range of applications in biological and biochemical research. In molecular biology, it is commonly used for the removal of RNA contaminants in DNA preparations. During DNA extraction, RNA can often co - purify with DNA, which can interfere with downstream applications such as PCR, sequencing, or cloning. By adding Ribonuclease A to the DNA sample, the RNA is degraded, leaving a pure DNA sample for further analysis.
In protein research, Ribonuclease A can be used to study the structure and function of ribonucleoprotein complexes. It can be used to disrupt the RNA - protein interactions, allowing for the isolation and analysis of the protein components. Additionally, it is used in the field of virology to study RNA viruses, as it can be used to degrade the viral RNA for further analysis.
First, reconstitute the lyophilized Ribonuclease A powder with an appropriate buffer. A common buffer for reconstitution is a Tris - HCl buffer at pH 7 - 8. The concentration of the reconstituted enzyme can be adjusted according to the specific application. For general RNA degradation in a DNA preparation, a final concentration of 10 - 100 μg/mL is usually sufficient.
Add the reconstituted Ribonuclease A to the sample containing RNA. Incubate the sample at the optimal temperature (usually 37°C) for a certain period of time, typically 15 - 30 minutes. After the incubation, the sample can be further processed according to the downstream application requirements. For example, if the next step is DNA purification, standard DNA purification protocols can be followed.
Case 1: DNA Extraction from Bacterial Cells. A research group was extracting genomic DNA from E. coli cells. After the initial cell lysis and DNA precipitation steps, they found that the DNA sample was contaminated with a significant amount of RNA. They added Ribonuclease A at a final concentration of 50 μg/mL to the DNA sample and incubated it at 37°C for 20 minutes. After this treatment, they performed agarose gel electrophoresis on the sample. The results showed that the RNA bands had disappeared, and the DNA was of high purity, which was suitable for subsequent PCR amplification and sequencing.
Case 2: Studying Ribonucleoprotein Complexes. Another group was interested in studying a ribonucleoprotein complex involved in mRNA splicing. They used Ribonuclease A to disrupt the RNA - protein interactions in the complex. After adding the enzyme and incubating at the optimal conditions, they were able to isolate the protein components of the complex using chromatography techniques. Further analysis of the proteins provided insights into their functions in the splicing process.
If you have any questions or need further assistance regarding this product, please feel free to contact us. Our email address is info@vivalr.com, and our phone number is (86) 15866781826.
Este é o William, Diretor Executivo da Zhishang Chemical Co., Ltd.
Bem-vindo a visitar a nossa fábrica. Como o principal fabricante de produtos químicos, temos nos esforçado para a qualidade do produto, inovação, P & D e atendimento ao cliente nos últimos 7 anos. Nos próximos 10 anos ou mais, estamos empenhados em nos tornarmos o fornecedor de produtos químicos mais confiável do mundo, criando uma marca internacional bem conhecida e de confiança dos clientes, com o espírito de "um metro de largura, dez mil metros de profundidade", continuamos a nos concentrar na pesquisa e desenvolvimento de produtos, continuamos a nos concentrar no atendimento ao cliente, continuamos a melhorar o sistema de serviço da cadeia de suprimentos, para criar uma equipe profissional de serviços de fornecimento de produtos químicos, para alcançar uma cooperação de longo prazo ganha-ganha. Por favor, não hesite em contactar-nos se tiver alguma dúvida.
Portuguese 
English (United States) 
English (Australia) 
Spanish