The product we are discussing here is Factory Supply Naringinase with CAS number 9068 - 31 - 9. CAS number is a unique numerical identifier assigned by the Chemical Abstracts Service to every chemical substance described in the open scientific literature. This specific naringinase is offered at the best price in the market, which is a great advantage for customers.
Naringinase is an enzyme that belongs to the class of hydrolases. It can catalyze the hydrolysis of naringin, a flavanone - 7 - O - glycoside. In terms of its physical and chemical properties, it usually exists in a powder form, which is easy to store and transport. The enzyme activity can be affected by factors such as temperature, pH value, and substrate concentration. Generally, it shows optimal activity at a certain temperature range (usually around 30 - 40°C) and pH value (around 4 - 6), but these values may vary depending on the specific manufacturing process and purity of the product.
1. Food Industry: In the food industry, naringinase plays a crucial role in debittering citrus juices. Naringin is a natural bitter - tasting compound found in citrus fruits. By adding naringinase to citrus juices, the naringin can be hydrolyzed into tasteless or less - bitter compounds, improving the taste of the juice. It can also be used in the production of flavonoid - rich food products, as it can help in the release of flavonoids from their glycosidic forms, enhancing the nutritional value and functionality of the food.
2. Pharmaceutical Industry: Naringin has certain biological activities, and the hydrolysis products of naringin by naringinase may have enhanced bioavailability and biological activities. It can be used in the development of pharmaceutical preparations to improve the solubility and absorption of naringin - related drugs. In addition, naringinase can also be used in the research of natural product chemistry for the structural modification and transformation of flavonoids.
1. Preparation: First, ensure that the naringinase powder is stored properly and is within the expiration date. Weigh the appropriate amount of naringinase according to the substrate concentration and the desired reaction degree. Usually, the dosage needs to be determined through preliminary experiments based on the specific application scenario.
2. Dissolution: Dissolve the weighed naringinase powder in a suitable buffer solution. The choice of buffer solution depends on the optimal pH value of the enzyme. For example, a citrate - phosphate buffer can be used for a pH range of 4 - 6. Stir gently to ensure complete dissolution.
3. Reaction: Add the dissolved naringinase solution to the substrate solution (such as citrus juice or naringin - containing solution). Mix well and maintain the reaction system at the optimal temperature. The reaction time also needs to be controlled according to the reaction progress, which can be monitored by detecting the change of naringin content using appropriate analytical methods.
4. Termination: After the reaction is completed, the reaction can be terminated by adjusting the temperature or pH value to inactivate the enzyme, or by separating the enzyme from the reaction system through appropriate separation methods.
Case 1: A citrus juice processing factory was facing the problem of bitter - tasting juice. They decided to use our Factory Supply Naringinase CAS 9068 - 31 - 9. First, they conducted a small - scale experiment. They took a sample of their citrus juice and added different amounts of naringinase at different temperatures and pH values. After testing, they found that adding 0.1% (w/v) of naringinase at 35°C and pH 5.5 for 2 hours could effectively reduce the bitterness of the juice. They then scaled up the process to their production line. After treatment with naringinase, the bitterness of the juice decreased significantly, and the sales of their juice increased by 20% within a month, which greatly improved the economic benefits of the factory.
Case 2: A pharmaceutical research institution was researching the bioavailability of a naringin - based drug. They used our naringinase to hydrolyze naringin into its more bioavailable form. By adding naringinase to the drug formulation and optimizing the reaction conditions, they found that the absorption rate of the drug in experimental animals increased by 30%. This result provided important support for the further development of the drug.
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Este é o William, Diretor Executivo da Zhishang Chemical Co., Ltd.
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