Octylphenylpolyethylene Glycol, commonly known as Triton X - 100, has the CAS number 9002 - 93 - 1. It is a non - ionic surfactant, which is a clear, colorless to pale yellow viscous liquid at room temperature. Triton X - 100 has a molecular formula that can vary slightly depending on the degree of ethoxylation, but typically it has an average of 9.5 ethylene oxide units per molecule.
It has a density of approximately 1.06 g/cm³ at 20°C and a boiling point above 200°C. The HLB (Hydrophile - Lipophile Balance) value of Triton X - 100 is around 13.5, which indicates its good balance between hydrophilic and lipophilic properties, making it highly effective in a wide range of applications.
Triton X - 100 is widely used in various industries. In the biological and biochemical fields, it is commonly used as a cell lysis agent. It can disrupt the cell membranes of both prokaryotic and eukaryotic cells, enabling the extraction of intracellular proteins, nucleic acids, and other biomolecules. In immunohistochemistry and immunofluorescence experiments, Triton X - 100 is used to permeabilize cell membranes, allowing antibodies to enter the cells and bind to the target antigens.
In the industrial cleaning sector, Triton X - 100 is an excellent detergent and emulsifier. It can effectively remove oils, greases, and dirt from various surfaces, including metal, glass, and plastic. It is also used in the formulation of paints and coatings to improve the dispersion of pigments and prevent aggregation. In the textile industry, it can be used as a wetting agent and leveling agent to ensure uniform dyeing of fabrics.
When using Triton X - 100 as a cell lysis agent in a biological laboratory, first, prepare a lysis buffer containing an appropriate concentration of Triton X - 100, usually around 0.1% - 1%. Then, add the lysis buffer to the cell suspension and incubate on ice for a certain period, typically 15 - 30 minutes. After incubation, centrifuge the mixture to separate the cell debris from the lysate.
In industrial cleaning applications, Triton X - 100 can be diluted with water at different ratios according to the degree of soiling. For light - soiled surfaces, a dilution ratio of 1:100 may be sufficient, while for heavy - soiled surfaces, a ratio of 1:10 or even higher may be required. The cleaning solution can be applied by spraying, wiping, or soaking, and then rinsed thoroughly with water.
In a research laboratory studying a novel protein, the scientists used Triton X - 100 for cell lysis. They grew a large quantity of recombinant E. coli cells expressing the target protein. By using a lysis buffer containing 0.5% Triton X - 100, they were able to efficiently break open the cells and release the protein. After subsequent purification steps, they obtained a high - yield and high - purity protein sample, which was crucial for their further structural and functional studies.
In an industrial setting, a metal parts manufacturing company was facing problems with oil and grease residues on their products. They decided to use a cleaning solution formulated with Triton X - 100. By diluting Triton X - 100 in water at a ratio of 1:20 and soaking the metal parts in the solution for 30 minutes, they were able to completely remove the contaminants. This not only improved the quality of their products but also reduced the production downtime caused by cleaning - related issues.
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Este é o William, Diretor Executivo da Zhishang Chemical Co., Ltd.
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