{"id":724,"date":"2025-03-07T12:05:46","date_gmt":"2025-03-07T12:05:46","guid":{"rendered":"https:\/\/vivalr.com\/product\/724.html"},"modified":"2025-03-07T12:23:16","modified_gmt":"2025-03-07T12:23:16","slug":"factory-supply-dotma-cas-104872-42-6-with-good-price","status":"publish","type":"product","link":"https:\/\/vivalr.com\/pt\/product\/724.html","title":{"rendered":"Factory Supply DOTMA CAS 104872-42-6 With Good Price"},"content":{"rendered":"<div class=\"product-guide\">\n<h1>DOTMA (CAS 104872-42-6) - High-Purity Gene Delivery Reagent<\/h1>\n<p>        <!-- Table of Contents --><\/p>\n<div class=\"toc\">\n<h2>\u00cdndice<\/h2>\n<ul>\n<li><a href=\"#product-overview\">1. Vis\u00e3o geral do produto<\/a><\/li>\n<li><a href=\"#key-applications\">2. Principais aplica\u00e7\u00f5es<\/a><\/li>\n<li><a href=\"#usage-instructions\">3. Instru\u00e7\u00f5es de utiliza\u00e7\u00e3o<\/a><\/li>\n<li><a href=\"#case-studies\">4. Estudos de caso<\/a><\/li>\n<li><a href=\"#contact\">5. Informa\u00e7\u00f5es de contacto<\/a><\/li>\n<\/ul><\/div>\n<p>        <!-- Product Overview --><\/p>\n<div id=\"product-overview\">\n<h2>1. Vis\u00e3o geral do produto<\/h2>\n<p><strong>DOTMA (CAS 104872-42-6)<\/strong> is a cationic lipid transfection reagent widely used for efficient gene delivery. Key specifications include:<\/p>\n<ul>\n<li><strong>Nome qu\u00edmico:<\/strong> N-[1-(2,3-Dioleyloxy)propyl]-N,N,N-trimethylammonium chloride<\/li>\n<li><strong>F\u00f3rmula molecular:<\/strong> C42H82ClNO2<\/li>\n<li><strong>Peso molecular:<\/strong> 668.57 g\/mol<\/li>\n<li><strong>Apar\u00eancia:<\/strong> P\u00f3 branco a esbranqui\u00e7ado<\/li>\n<li><strong>Pureza:<\/strong> \u226598% (HPLC)<\/li>\n<li><strong>Solubilidade:<\/strong> Soluble in chloroform, ethanol, and DMSO<\/li>\n<li><strong>Armazenamento:<\/strong> Store at -20\u00b0C in airtight containers<\/li>\n<\/ul><\/div>\n<p>        <!-- Key Applications --><\/p>\n<div id=\"key-applications\">\n<h2>2. Principais aplica\u00e7\u00f5es<\/h2>\n<p>DOTMA is a critical reagent for:<\/p>\n<ul>\n<li><strong>Terapia gen\u00e9tica:<\/strong> Non-viral vector for DNA\/RNA delivery in vitro and in vivo.<\/li>\n<li><strong>Molecular Biology:<\/strong> Transfection of plasmids, siRNA, and mRNA into mammalian cells.<\/li>\n<li><strong>Desenvolvimento de medicamentos:<\/strong> Screening gene-based therapeutics in research models.<\/li>\n<li><strong>Investiga\u00e7\u00e3o acad\u00e9mica:<\/strong> Studying gene expression and cellular pathways.<\/li>\n<\/ul><\/div>\n<p>        <!-- Usage Instructions --><\/p>\n<div id=\"usage-instructions\">\n<h2>3. Instru\u00e7\u00f5es de utiliza\u00e7\u00e3o<\/h2>\n<ol>\n<li><strong>Prepara\u00e7\u00e3o:<\/strong> Dissolve DOTMA in ethanol (10 mg\/mL) and store at -20\u00b0C.<\/li>\n<li><strong>Complex Formation:<\/strong> Mix DOTMA with nucleic acids at a 3:1 ratio (v\/v) in serum-free media.<\/li>\n<li><strong>Incubation:<\/strong> Allow 20-30 minutes for lipid-nucleic acid complex formation.<\/li>\n<li><strong>Transfection:<\/strong> Add complexes to cells and incubate for 4-6 hours before replacing media.<\/li>\n<li><strong>Analysis:<\/strong> Assess gene expression 24-72 hours post-transfection.<\/li>\n<\/ol>\n<p><em>Nota:<\/em> Optimize ratios and incubation times for specific cell types.<\/p>\n<\/p><\/div>\n<p>        <!-- Case Studies --><\/p>\n<div id=\"case-studies\">\n<h2>4. Estudos de caso<\/h2>\n<h3>Case 1: In Vitro Transfection of HEK293 Cells<\/h3>\n<ol>\n<li>HEK293 cells were seeded at 70% confluency in 6-well plates.<\/li>\n<li>DOTMA (20 \u03bcg) was combined with 5 \u03bcg of GFP plasmid DNA.<\/li>\n<li>Transfection efficiency reached 85% after 48 hours, confirmed via fluorescence microscopy.<\/li>\n<\/ol>\n<h3>Case 2: siRNA Delivery for Gene Knockdown<\/h3>\n<ol>\n<li>DOTMA-mediated delivery of 50 nM siRNA targeting oncogene KRAS in A549 cells.<\/li>\n<li>Observed 70% reduction in KRAS mRNA levels via qPCR after 72 hours.<\/li>\n<\/ol><\/div>\n<p>        <!-- Contact --><\/p>\n<div id=\"contact\">\n<h2>5. Informa\u00e7\u00f5es de contacto<\/h2>\n<p>Para encomendas por grosso ou pedidos de informa\u00e7\u00e3o t\u00e9cnica:<\/p>\n<ul>\n<li>Correio eletr\u00f3nico: <a href=\"mailto:info@vivalr.com\">info@vivalr.com<\/a><\/li>\n<li>Telefone: <a href=\"tel:+8615866781826\">(86) 15866781826<\/a><\/li>\n<\/ul><\/div>\n<\/p><\/div>","protected":false},"excerpt":{"rendered":"<p>DOTMA (CAS 104872-42-6) - High-Purity Gene Delivery Reagent Table of Contents 1. Product Overview 2. Key Applications 3. Usage Instructions 4. Case Studies 5. Contact Information 1. Product Overview DOTMA (CAS 104872-42-6) is a cationic lipid transfection reagent widely used for efficient gene delivery. Key specifications include: Chemical Name: N-[1-(2,3-Dioleyloxy)propyl]-N,N,N-trimethylammonium chloride Molecular Formula: C42H82ClNO2 Molecular [&hellip;]<\/p>","protected":false},"featured_media":21527,"comment_status":"closed","ping_status":"open","template":"","meta":[],"product_brand":[],"product_cat":[15],"product_tag":[],"class_list":{"0":"post-724","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-uncategorized","8":"first","9":"instock","10":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v24.4 (Yoast SEO v27.4) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Factory Supply DOTMA CAS 104872-42-6 With Good Price - Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/vivalr.com\/pt\/product\/724.html\" \/>\n<meta property=\"og:locale\" content=\"pt_PT\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Factory Supply DOTMA CAS 104872-42-6 With Good Price\" \/>\n<meta property=\"og:description\" content=\"DOTMA (CAS 104872-42-6) - High-Purity Gene Delivery Reagent Table of Contents 1. Product Overview 2. Key Applications 3. Usage Instructions 4. Case Studies 5. Contact Information 1. Product Overview DOTMA (CAS 104872-42-6) is a cationic lipid transfection reagent widely used for efficient gene delivery. Key specifications include: Chemical Name: N-[1-(2,3-Dioleyloxy)propyl]-N,N,N-trimethylammonium chloride Molecular Formula: C42H82ClNO2 Molecular [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/vivalr.com\/pt\/product\/724.html\" \/>\n<meta property=\"og:site_name\" content=\"Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01\" \/>\n<meta property=\"article:modified_time\" content=\"2025-03-07T12:23:16+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/vivalr.com\/wp-content\/uploads\/2025\/03\/DOTMA-CAS-104872-42-6.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1165\" \/>\n\t<meta property=\"og:image:height\" content=\"1163\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Tempo estimado de leitura\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minuto\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/724.html\",\"url\":\"https:\\\/\\\/vivalr.com\\\/product\\\/724.html\",\"name\":\"Factory Supply DOTMA CAS 104872-42-6 With Good Price - Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/vivalr.com\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/724.html#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/724.html#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/vivalr.com\\\/wp-content\\\/uploads\\\/2025\\\/03\\\/DOTMA-CAS-104872-42-6.jpg\",\"datePublished\":\"2025-03-07T12:05:46+00:00\",\"dateModified\":\"2025-03-07T12:23:16+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/724.html#breadcrumb\"},\"inLanguage\":\"pt-PT\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/vivalr.com\\\/product\\\/724.html\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"pt-PT\",\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/724.html#primaryimage\",\"url\":\"https:\\\/\\\/vivalr.com\\\/wp-content\\\/uploads\\\/2025\\\/03\\\/DOTMA-CAS-104872-42-6.jpg\",\"contentUrl\":\"https:\\\/\\\/vivalr.com\\\/wp-content\\\/uploads\\\/2025\\\/03\\\/DOTMA-CAS-104872-42-6.jpg\",\"width\":1165,\"height\":1163},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/vivalr.com\\\/product\\\/724.html#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/vivalr.com\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Shop\",\"item\":\"https:\\\/\\\/vivalr.com\\\/shop\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Factory Supply DOTMA CAS 104872-42-6 With Good Price\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/vivalr.com\\\/#website\",\"url\":\"https:\\\/\\\/vivalr.com\\\/\",\"name\":\"Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01\",\"description\":\"Discover a wide range of high-quality chemical raw materials to meet your industrial needs. Our commitment to excellence ensures that you get the best products and services.\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/vivalr.com\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"pt-PT\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Factory Supply DOTMA CAS 104872-42-6 With Good Price - Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/vivalr.com\/pt\/product\/724.html","og_locale":"pt_PT","og_type":"article","og_title":"Factory Supply DOTMA CAS 104872-42-6 With Good Price","og_description":"DOTMA (CAS 104872-42-6) - High-Purity Gene Delivery Reagent Table of Contents 1. Product Overview 2. Key Applications 3. Usage Instructions 4. Case Studies 5. Contact Information 1. Product Overview DOTMA (CAS 104872-42-6) is a cationic lipid transfection reagent widely used for efficient gene delivery. Key specifications include: Chemical Name: N-[1-(2,3-Dioleyloxy)propyl]-N,N,N-trimethylammonium chloride Molecular Formula: C42H82ClNO2 Molecular [&hellip;]","og_url":"https:\/\/vivalr.com\/pt\/product\/724.html","og_site_name":"Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01","article_modified_time":"2025-03-07T12:23:16+00:00","og_image":[{"width":1165,"height":1163,"url":"https:\/\/vivalr.com\/wp-content\/uploads\/2025\/03\/DOTMA-CAS-104872-42-6.jpg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","twitter_misc":{"Tempo estimado de leitura":"1 minuto"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/vivalr.com\/product\/724.html","url":"https:\/\/vivalr.com\/product\/724.html","name":"Factory Supply DOTMA CAS 104872-42-6 With Good Price - Welcome to VIVALR- Your Trusted Source for Chemical Raw Materials\uff01","isPartOf":{"@id":"https:\/\/vivalr.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/vivalr.com\/product\/724.html#primaryimage"},"image":{"@id":"https:\/\/vivalr.com\/product\/724.html#primaryimage"},"thumbnailUrl":"https:\/\/vivalr.com\/wp-content\/uploads\/2025\/03\/DOTMA-CAS-104872-42-6.jpg","datePublished":"2025-03-07T12:05:46+00:00","dateModified":"2025-03-07T12:23:16+00:00","breadcrumb":{"@id":"https:\/\/vivalr.com\/product\/724.html#breadcrumb"},"inLanguage":"pt-PT","potentialAction":[{"@type":"ReadAction","target":["https:\/\/vivalr.com\/product\/724.html"]}]},{"@type":"ImageObject","inLanguage":"pt-PT","@id":"https:\/\/vivalr.com\/product\/724.html#primaryimage","url":"https:\/\/vivalr.com\/wp-content\/uploads\/2025\/03\/DOTMA-CAS-104872-42-6.jpg","contentUrl":"https:\/\/vivalr.com\/wp-content\/uploads\/2025\/03\/DOTMA-CAS-104872-42-6.jpg","width":1165,"height":1163},{"@type":"BreadcrumbList","@id":"https:\/\/vivalr.com\/product\/724.html#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/vivalr.com\/"},{"@type":"ListItem","position":2,"name":"Shop","item":"https:\/\/vivalr.com\/shop\/"},{"@type":"ListItem","position":3,"name":"Factory Supply DOTMA CAS 104872-42-6 With Good Price"}]},{"@type":"WebSite","@id":"https:\/\/vivalr.com\/#website","url":"https:\/\/vivalr.com\/","name":"Bem-vindo ao VIVALR- Sua fonte confi\u00e1vel de mat\u00e9rias-primas qu\u00edmicas\uff01","description":"Descubra uma vasta gama de mat\u00e9rias-primas qu\u00edmicas de alta qualidade para satisfazer as suas necessidades industriais. O nosso compromisso com a excel\u00eancia garante-lhe os melhores produtos e servi\u00e7os.","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/vivalr.com\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"pt-PT"}]}},"_links":{"self":[{"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/product\/724","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/comments?post=724"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/media\/21527"}],"wp:attachment":[{"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/media?parent=724"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/product_brand?post=724"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/product_cat?post=724"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/vivalr.com\/pt\/wp-json\/wp\/v2\/product_tag?post=724"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}