User Guide for High Quality PUROMYCIN DIHYDROCHLORIDE CAS 58-58-2

Table of Contents

• 1. Basic Information and Parameters
• 2. Applications
• 3. Usage
• 4. Case Studies
• 5. Contact Information

1. Basic Information and Parameters

PUROMYCIN DIHYDROCHLORIDE, with CAS number 58 - 58 - 2, is a high - quality product offered at the best price. It is an aminonucleoside antibiotic. The chemical formula of puromycin dihydrochloride is C₁₇H₂₃N₇O₅·2HCl. It has a molecular weight of approximately 494.33 g/mol.

The product is typically presented as a white to off - white powder. It is highly soluble in water, which makes it convenient for various experimental and industrial applications. The purity of our high - quality puromycin dihydrochloride meets strict standards, ensuring reliable and reproducible results in different uses.

2. Applications

PUROMYCIN DIHYDROCHLORIDE has a wide range of applications in the fields of biological research and biotechnology.

In cell biology, it is commonly used as a selective agent in cell culture. It can inhibit protein synthesis in both prokaryotic and eukaryotic cells by incorporating into the growing polypeptide chain and causing premature chain termination. This property allows researchers to select for cells that have been successfully transfected with a gene conferring puromycin resistance, which is often used in gene expression studies, gene knockout experiments, and the generation of stable cell lines.

In microbiology, it can be used to study the mechanism of protein synthesis in bacteria and other microorganisms. It helps in understanding the fundamental processes of translation and the role of ribosomes.

3. Usage

When using PUROMYCIN DIHYDROCHLORIDE in cell culture, it is crucial to prepare an appropriate stock solution. First, dissolve the powder in sterile water to make a stock solution with a concentration of 10 - 100 mg/mL. This stock solution should be stored at - 20°C to maintain its stability.

Before adding it to the cell culture medium, the stock solution needs to be diluted to the appropriate working concentration. The optimal working concentration depends on the cell type and the specific experiment. Generally, a working concentration of 1 - 10 μg/mL is used for most mammalian cell lines.

When adding the puromycin to the cell culture, make sure to mix it well with the medium to ensure uniform distribution. Monitor the cells regularly during the selection process. Cells that are sensitive to puromycin will start to die within 24 - 48 hours, while resistant cells will continue to grow.

4. Case Studies

Case Study 1: A research team was working on a project to generate a stable cell line expressing a specific protein. They transfected the cells with a plasmid containing the gene of interest along with a puromycin resistance gene. After transfection, they added puromycin dihydrochloride to the cell culture medium at a working concentration of 2 μg/mL. Within 48 hours, the non - transfected cells started to die, and after a week of selection, only the cells that had successfully incorporated the plasmid were left. These cells were then expanded and used for further protein expression studies.

Case Study 2: In a microbiology lab, researchers were studying the translation mechanism in E. coli. They added puromycin dihydrochloride to the bacterial culture at a specific concentration. By observing the changes in protein synthesis patterns, they were able to gain insights into the role of the ribosome in polypeptide chain elongation and termination.

5. Contact Information

If you have any questions or need further information about High Quality PUROMYCIN DIHYDROCHLORIDE CAS 58 - 58 - 2, please feel free to contact us. Our email is info@vivalr.com, and our phone number is (86) 15866781826.