User Guide: Factory Supply β-Galactosidase CAS 9031-11-2 With Good Price

Table of Contents

• Basic Information and Parameters
• Product Uses
• Product Usage
• Usage Cases
• Contact Information

Basic Information and Parameters

Our Factory Supply β-Galactosidase, identified by the CAS number 9031 - 11 - 2, is a high - quality enzyme product. β - Galactosidase, also known as lactase, is an enzyme that catalyzes the hydrolysis of β - galactosides into monosaccharides.

Parameters:

• Appearance: Usually appears as a white to light - yellow powder.
• Activity: The enzyme activity can vary depending on the specific product formulation. Generally, it has high catalytic activity under appropriate conditions.
• Solubility: It is soluble in water, which makes it convenient for various applications.
• Optimal pH: The optimal pH range for its activity is typically around 6.0 - 7.0, but this can vary slightly depending on the source and purification process.
• Optimal Temperature: The enzyme shows the best activity at a temperature range of 35 - 40°C.

Product Uses

β - Galactosidase has a wide range of applications in different industries:

• Dairy Industry: It is used to hydrolyze lactose in milk and dairy products. This is particularly useful for lactose - intolerant individuals. By breaking down lactose into glucose and galactose, it improves the digestibility of dairy products and also enhances their sweetness.
• Food Industry: In addition to the dairy sector, it can be used in the production of other food items such as ice cream and yogurt. It helps to prevent the crystallization of lactose, resulting in a smoother texture of the final products.
• Biochemical Research: In laboratories, β - Galactosidase is a commonly used tool in molecular biology. It can be used as a reporter gene in gene expression studies. The enzyme's activity can be easily detected using specific substrates, which makes it a valuable marker for gene function analysis.
• Pharmaceutical Industry: It can be used in the development of drugs and formulations. For example, it can be used to modify the structure of certain glycosides for better drug delivery and efficacy.

Product Usage

When using β - Galactosidase, the following steps should be followed:

• Reconstitution: If the product is in powder form, it should be reconstituted in an appropriate buffer solution. The choice of buffer depends on the specific application and the optimal pH requirements of the enzyme. For most general applications, a phosphate - buffered saline (PBS) at pH 7.0 can be used.
• Dosage: The dosage of the enzyme depends on the substrate concentration and the desired reaction rate. In the dairy industry, for example, a typical dosage might range from 0.1 - 1.0 grams per liter of milk, depending on the lactose content and the processing time.
• Reaction Conditions: The reaction should be carried out at the optimal temperature and pH for the enzyme. The reaction mixture should be gently stirred to ensure uniform distribution of the enzyme and the substrate.
• Termination of Reaction: Once the desired degree of hydrolysis is achieved, the reaction can be terminated by adjusting the pH or temperature to a non - optimal range for the enzyme activity.

Usage Cases

Case 1: Dairy Product for Lactose - Intolerant Consumers

A dairy company wanted to produce lactose - free milk. They added our β - Galactosidase to fresh milk at a dosage of 0.5 grams per liter. The milk was then incubated at 37°C for 4 hours with gentle stirring. After the reaction, the lactose content in the milk was reduced to undetectable levels, as measured by a lactose assay. The resulting lactose - free milk was well - received by lactose - intolerant consumers, and the company saw an increase in sales.

Case 2: Biochemical Research on Gene Expression

A research laboratory was studying the expression of a particular gene in bacteria. They inserted the β - Galactosidase gene as a reporter gene downstream of the gene of interest. When