Factory Supply Hematoxylin CAS 517-28-2 With Best Price: User Guide

Table of Contents

• 1. Basic Information and Parameters
• 2. Product Uses
• 3. How to Use the Product
• 4. Product Use Cases
• 5. Contact Information

1. Basic Information and Parameters

The product, Factory Supply Hematoxylin CAS 517 - 28 - 2, is hematoxylin, a natural dye with a CAS number of 517 - 28 - 2. Hematoxylin is derived from the heartwood of the logwood tree (Haematoxylum campechianum). It has the chemical formula C₁₆H₁₄O₆ and a molecular weight of approximately 302.28 g/mol. This compound is a crystalline solid, usually in the form of pale yellow to light brown crystals. It is slightly soluble in cold water but more soluble in hot water, alcohol, and ether. The purity of our factory - supplied hematoxylin is high, ensuring consistent performance in various applications.

2. Product Uses

Hematoxylin is widely used in histology and cytology laboratories. It is one of the most important dyes in histological staining. When combined with an appropriate mordant, it can stain cell nuclei blue - purple. This staining allows for clear visualization of the nuclear structure under a microscope, which is crucial for the diagnosis of diseases such as cancer. In addition to histology, hematoxylin is also used in the field of microscopy for staining plant tissues, enabling researchers to study plant cell structures. It can also be used in the manufacturing of inks and as a natural colorant in some industries.

3. How to Use the Product

When using hematoxylin for histological staining, the following steps are generally followed:

1. Prepare the tissue sections: The tissue samples need to be properly fixed, dehydrated, and embedded in paraffin. Then, thin sections are cut and mounted on glass slides.
2. Deparaffinize and rehydrate the sections: Use xylene to remove the paraffin from the sections, followed by a series of graded alcohols to rehydrate the tissue.
3. Prepare the hematoxylin solution: Dissolve hematoxylin in an appropriate solvent, usually water or alcohol, and add a mordant such as alum. The solution may need to be ripened for a certain period to achieve the best staining effect.
4. Stain the sections: Immerse the rehydrated tissue sections in the hematoxylin solution for a specific time, usually 5 - 10 minutes. The staining time may need to be adjusted according to the type of tissue and the desired staining intensity.
5. Rinse and differentiate: Rinse the stained sections with water to remove the excess hematoxylin. Then, use a differentiating solution, such as acid alcohol, to selectively remove the hematoxylin from non - nuclear areas, enhancing the contrast of the staining.
6. Counterstain (optional): If necessary, a counterstain can be used to stain the cytoplasm or other cellular components, such as eosin.
7. Dehydrate and mount: After staining, the sections are dehydrated through a series of graded alcohols and cleared with xylene. Finally, a coverslip is mounted on the section using a mounting medium.

4. Product Use Cases

Case 1: Cancer Diagnosis in a Pathology Laboratory
In a large - scale pathology laboratory, hematoxylin staining was used for the diagnosis of breast cancer. The pathologists prepared tissue sections from breast tumor samples. After staining with hematoxylin and eosin (H&E staining), they were able to clearly observe the nuclear morphology and structure of the cancer cells. The hematoxylin - stained nuclei appeared blue - purple, allowing for the identification of abnormal nuclear features such as enlarged nuclei, irregular nuclear membranes, and increased nuclear - to - cytoplasmic ratios. This staining method played a crucial role in the accurate diagnosis and grading of breast cancer, guiding the subsequent treatment decisions.

Case 2: Plant Tissue Research in a Botanical Laboratory
In a botanical research laboratory, researchers were studying the structure of plant roots. They used hematoxylin to stain the root tissue sections. By staining the cell nuclei, they could clearly distinguish different cell layers in the root, such as the epidermis, cortex, and vascular cylinder. This staining technique helped them understand the growth and development processes of plant roots and provided important information for plant breeding and agricultural research.