Tris(hydroxymethyl)aminomethane hydrochloride, with the CAS number 1185-53-1, is a commonly used biological buffer. It is a white crystalline powder with high solubility in water. The molecular formula of tris(hydroxymethyl)aminomethane hydrochloride is C₄H₁₂ClNO₃, and its molecular weight is approximately 157.6 g/mol. This product is provided by our factory with a good price, ensuring high - quality and cost - effective supply.
The purity of our tris(hydroxymethyl)aminomethane hydrochloride is usually above 99%, which meets the strict requirements of various scientific research and industrial applications. It has a pH range of 7.0 - 9.0 in a 0.1M aqueous solution at 25°C, making it suitable for a wide variety of buffer systems.
2.1 Biological Research
In biological research, tris(hydroxymethyl)aminomethane hydrochloride is widely used as a buffer in biochemical reactions. It can maintain a stable pH environment for enzymes, proteins, and nucleic acids, ensuring the normal progress of biochemical reactions such as polymerase chain reaction (PCR), enzyme - linked immunosorbent assay (ELISA), and western blotting.
2.2 Pharmaceutical Industry
In the pharmaceutical industry, it is used in the formulation of drugs. It can be used to adjust the pH of drug solutions, improve the stability and solubility of drugs, and enhance the effectiveness and safety of drugs.
2.3 Cosmetics Industry
It can also be used in the cosmetics industry. It helps to adjust the pH of cosmetic products, making them more suitable for the skin's pH range and reducing skin irritation.
3.1 Preparation of Buffer Solution
To prepare a tris(hydroxymethyl)aminomethane hydrochloride buffer solution, first, accurately weigh the appropriate amount of tris(hydroxymethyl)aminomethane hydrochloride according to the required concentration. Dissolve it in deionized water. Then, use hydrochloric acid or sodium hydroxide to adjust the pH to the desired value. Finally, adjust the volume to the final volume with deionized water.
3.2 Storage Conditions
This product should be stored in a cool, dry place, away from light and heat. It should be kept in a tightly closed container to prevent moisture and contamination. When using, avoid long - term exposure to air to prevent oxidation and degradation.
4.1 PCR Experiment in a Research Institute
A well - known research institute was conducting a PCR experiment to amplify a specific DNA fragment. They used a tris(hydroxymethyl)aminomethane hydrochloride buffer solution at a concentration of 10 mM with a pH of 8.3. The buffer provided a stable pH environment for the Taq polymerase and other reaction components. As a result, the PCR reaction was highly efficient, and a large amount of the target DNA fragment was successfully amplified. The researchers found that the high - quality tris(hydroxymethyl)aminomethane hydrochloride they obtained from our factory contributed significantly to the success of the experiment.
4.2 Drug Formulation in a Pharmaceutical Company
A pharmaceutical company was developing a new injectable drug. They used tris(hydroxymethyl)aminomethane hydrochloride to adjust the pH of the drug solution. By carefully formulating the buffer system, they improved the solubility and stability of the drug. After a series of tests, the drug showed better efficacy and fewer side effects. The company was satisfied with the performance of our tris(hydroxymethyl)aminomethane hydrochloride in the drug formulation process.
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