The product we are introducing is bis - tris with CAS number 6976 - 37 - 0. It is a high - quality chemical compound supplied directly from the factory at a low price. Bis - tris, chemically known as 2 - [Bis(2 - hydroxyethyl)amino] - 2 - (hydroxymethyl)propane - 1,3 - diol, is a white crystalline powder. It has a molecular formula of C₈H₁₉NO₅ and a molecular weight of approximately 209.24 g/mol. The compound is soluble in water, and its pH value in a 0.1 M solution is usually in the range of 6.5 - 9.0 at 25°C. It has good stability under normal storage conditions (cool, dry place) and can maintain its chemical properties for a relatively long time.
1. Biochemical research: Bis - tris is widely used as a buffer in biochemical experiments. It can maintain a stable pH environment, which is crucial for the normal progress of various enzymatic reactions, protein purification, and DNA/RNA electrophoresis. For example, in protein purification processes, maintaining the appropriate pH can prevent protein denaturation and ensure the activity of the target protein.
2. Pharmaceutical industry: It can be used as an excipient in some drug formulations to adjust the pH of the drug solution, improve the stability and solubility of the drug, and enhance the bioavailability of the drug.
3. Cosmetics: In the cosmetics industry, bis - tris can be used to adjust the pH of skin - care products, making them more suitable for the skin's pH range, and improving the comfort and effectiveness of the products.
1. Preparation of buffer solution: First, weigh an appropriate amount of bis - tris according to the required concentration. For example, if you want to prepare a 0.1 M bis - tris buffer solution, calculate the mass based on the molecular weight. Dissolve the weighed bis - tris in a small amount of distilled water. Then, use a pH meter to monitor the pH value, and adjust it to the desired range by adding an appropriate amount of acid or base (usually hydrochloric acid or sodium hydroxide). Finally, dilute the solution to the required volume with distilled water.
2. In pharmaceutical and cosmetic applications: Follow the relevant production processes and formulas. When using bis - tris as an excipient in drugs or cosmetics, ensure that it is evenly mixed with other ingredients under appropriate conditions to achieve the best results.
1. Biochemical research case: A research team was conducting a study on a specific enzyme. They used a bis - tris buffer solution with a pH of 7.5 to maintain a stable environment for the enzymatic reaction. By using this buffer, they found that the enzyme activity was significantly improved compared to using other buffers. The reaction rate was faster, and the yield of the reaction product was higher. The stable pH provided by bis - tris ensured the normal conformation and activity of the enzyme, which was crucial for the success of the experiment.
2. Pharmaceutical application case: A pharmaceutical company was developing a new injectable drug. They used bis - tris to adjust the pH of the drug solution. After adding bis - tris, the drug showed better stability during storage. The degradation rate of the active ingredient was significantly reduced, and the solubility of the drug was improved. This not only extended the shelf - life of the drug but also enhanced its therapeutic effect.
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