DIPSO (Cas 68399-80-4) User Guide: Specifications, Uses, and Applications
Table of Contents
1. Product Specifications
Product Name: DIPSO (3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid)
CAS Number: 68399-80-4
Molecular Formula: C7H17NO6S
Molecular Weight: 243.28 g/mol
Appearance: White crystalline powder
Purity: ≥99% (HPLC)
Storage: Keep in a dry, cool place (2-8°C), protected from light.
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2. Key Uses of DIPSO
DIPSO is a high-purity biochemical buffer widely used in:
- Cell culture media to maintain stable pH (6.8-8.2).
- Protein purification and enzyme studies.
- Diagnostic reagent formulations.
- Molecular biology experiments requiring low-metal ion content.
Its low toxicity and compatibility with biological systems make it ideal for sensitive applications.
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3. How to Use DIPSO
Step-by-Step Guide:
- Preparation: Dissolve in distilled water at 25°C (typical concentration: 0.1-0.5M).
- pH Adjustment: Use NaOH/HCl to reach desired pH (verify with calibrated meter).
- Sterilization: Autoclave at 121°C for 20 minutes or filter through 0.22µm membrane.
- Storage: Store prepared solutions at 4°C for ≤1 month.
Safety: Wear gloves and goggles; avoid inhalation. Dispose per local regulations.
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4. Detailed Application Cases
Case 1: Mammalian Cell Culture
Used at 25mM concentration in CHO cell media, maintaining pH 7.4±0.1 for 72 hours, improving cell viability by 18% vs. traditional buffers.
Case 2: Protein Crystallography
Enabled stable pH 7.8 environment during lysozyme crystallization, achieving 92% crystal clarity in 24 hours.
Case 3: IVD Reagent Production
Critical component in COVID-19 PCR test kits, demonstrating ≤0.1% lot-to-lot variability in buffer performance.
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