High Quality Glutamate Dehydrogenase (CAS 9029-12-3 / 9029-11-2) - Product Guide

1. Product Information
2. Applications & Uses
3. Usage Instructions
4. Detailed Use Cases

1. Product Information

Product Name: Glutamate Dehydrogenase (GDH)

CAS Number: 9029-12-3 / 9029-11-2

Source: Recombinant expression from microbial strains

Purity: ≥95% (SDS-PAGE)

Activity: ≥200 units/mg protein

Storage: Stable at 2-8°C for 24 months; avoid freeze-thaw cycles

2. Applications & Uses

Glutamate Dehydrogenase is a critical enzyme widely used in:

Diagnostic Kits: Detection of ammonia levels in blood/plasma.
Biochemical Research: Study of amino acid metabolism and enzyme kinetics.
Biotechnology: NAD(P)H regeneration systems in industrial biocatalysis.
Food Safety Testing: Quantification of glutamate in processed foods.

3. Usage Instructions

Step-by-Step Protocol:

Reconstitution: Dilute lyophilized GDH in 0.1M phosphate buffer (pH 7.4).
Sample Preparation: Centrifuge biological samples at 10,000×g for 5 minutes.
Reaction Setup: Mix 50µL sample with 100µL assay buffer, 10µL NAD⁺ (2mM), and 10µL enzyme.
Measurement: Monitor absorbance at 340nm for NADH formation (25°C, 5 minutes).
Calculation: Use extinction coefficient ε₃₄₀ = 6.22 mM⁻¹cm⁻¹ for quantification.

Note: Optimize enzyme concentration based on substrate load.

4. Detailed Use Cases

Case Study 1: Blood Ammonia Detection

Application: Diagnosing hepatic encephalopathy in clinical settings.

Procedure:

Add 10µL plasma to 200µL reaction mix (containing α-ketoglutarate and NADH).
Incubate at 37°C for 15 minutes.
Measure NADH depletion rate at 340nm.

Result: Linear range 10-500µM ammonia with 98% correlation to reference methods.

Case Study 2: Glutamate Biosensor Development

Application: Real-time monitoring in fermentation processes.

Procedure:

Immobilize GDH on carbon nanotube electrodes.
Calibrate using 0.1-10mM glutamate standards.
Measure current change via NADH oxidation.

Result: 90% signal stability after 50 operational cycles.

Contact Us

Email: info@vivalr.com

Phone: (86) 15866781826

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About the company

Vivalr, a paragon of innovation in the chemical sector, fuses cutting - edge R & D with unwavering commitment to quality. Our product spectrum, from industrial stalwarts to fine - tuned chemicals, is a testament to our versatility.

Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
2000 square R&D center
More than 20 research and development personnel,
12 sets of research and development equipment, 10 fume hoods and 6 university laboratories have been developed in cooperation
18 patents, 140 sets of chemical synthesis processes

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
For the United States, Australia, Japan, South Korea, Sweden, France, Spain and other global more than 2000 customers to provide high-quality products and services, has won the recognition and trust of customers in the industry.

More than 12 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

Mode of Transportation

By Air, fast but expensive.

By Sea, usual and economy.

By Train, suit for middle Asia countries.

FAQ

Does it support auditing?

Environmental Protection Science and Technology Park, Zhengfeng Road, High-tech Zone, Jinan City, Shandong Province

Whether to provide samples?

Free samples can be provided, and customers only need to pay the corresponding shipping costs

Delivery time

There are stock in the warehouse, and some products need to be customized for production within 3 working days, depending on the workshop situation and production schedule

What payment methods and currencies are accepted here?

We accept payment methods such as TT, Western Union, and letter of credit, etc. Meanwhile, we support currencies like US dollars, euros, RMB, and Bitcoin, etc.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Vivalr’s delivery rate is guaranteed to be 99.99%

2000+

square meter R&D center

12+

China(set) of R&D equipment

10+

Fume hood

20+

R&D team

User’evaluation of us

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Marcus Long

" In years of sourcing from China, first found working with Sincere Chemical great. They help in production, delivery and tech, saving time. When first asking Zhai for optical brightener quote, got detailed one with prices, trade details, company info, R&D and production capacity, packaging and raw material control."

Emma Kung

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Lisa Phoenix

" Sincere Chemical team visited our USA factory in the year of 2017. We think highly of cooperation with them. We thanked you for the past excellent orders and best product quality. We also talked about some new products for future orders. We will be still in strategic cooperation in the future and help each other."

Marcus Long

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Emma Kung

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