ACES (CAS 7365-82-4), chemically known as 2-[(2-Amino-2-oxoethyl)amino]ethanesulfonic acid, is a high-purity biological buffer widely used in biochemical and pharmaceutical research. Key specifications include:
ACES is a zwitterionic buffer ideal for:
Its low toxicity, minimal metal ion binding, and compatibility with biological systems make it a preferred choice for sensitive applications.
ACES buffer (20 mM, pH 7.0) was used to maintain pH stability in mammalian cell cultures, enhancing cell viability by 30% compared to traditional buffers.
A 40 mM ACES-based running buffer (pH 6.9) improved resolution of DNA fragments ≤500 bp in agarose gels, reducing smearing effects.
ACES (50 mM, pH 7.2) preserved 95% of lactate dehydrogenase activity after 24 hours at 25°C, outperforming phosphate buffers.
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