Specialized inHigh Quality 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALT CAS 102185-33-1 With Good Priceand related product development and production

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1. Product Information: 5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt (CAS 102185-33-1)

Chemical Name: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt

CAS Number: 102185-33-1

Molecular Formula: C₈H₄BrClNNa₂O₄P

Molecular Weight: 393.43 g/mol

Appearance: White to off-white crystalline powder

Purity: ≥98% (HPLC)

Storage: Store at -20°C in a dry, dark environment

Solubility: Soluble in water, DMSO, and aqueous buffers

2. Applications of 5-Bromo-4-chloro-3-indolyl Phosphate Disodium Salt

This substrate is widely used in biochemical assays for detecting alkaline phosphatase (ALP) activity. Key applications include:

Western blotting for chromogenic detection of ALP-conjugated antibodies
ELISA (Enzyme-Linked Immunosorbent Assay) applications
Histochemical staining in cell and tissue samples
DNA/RNA hybridization detection systems

When combined with Nitro Blue Tetrazolium (NBT), it produces an insoluble blue precipitate, enabling precise localization of enzymatic activity.

3. Step-by-Step Usage Protocols

Solution Preparation: Dissolve in deionized water to 1 mg/mL as a stock solution (store at -20°C for ≤6 months).
Working Solution: Mix with NBT in a 1:1 ratio in alkaline phosphatase buffer (pH 9.5).
Incubation: Apply to membranes/cells at 25°C for 5-30 minutes (optimize based on signal intensity).
Reaction Stop: Rinse thoroughly with distilled water or EDTA-containing buffer.

Note: Protect from light during all steps to prevent photodegradation.

4. Practical Case Studies

Case 1: Western Blot Detection

Researchers successfully identified GFP-tagged proteins by combining this substrate with ALP-conjugated secondary antibodies. Visible blue bands developed within 8 minutes at 22°C, demonstrating high sensitivity with 0.1 ng detectable protein.

Case 2: Tissue Section Analysis

In murine kidney studies, a 15-minute incubation at 37°C revealed precise tubular ALP distribution. The chromogen showed 40% higher resolution compared to alternative substrates in parallel tests.

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This is William, CEO of Zhishang Chemical Co., Ltd.

Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.

Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
2000 square R&D center
More than 20 research and development personnel,
12 sets of research and development equipment, 10 fume hoods and 6 university laboratories have been developed in cooperation
18 patents, 140 sets of chemical synthesis processes

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
For the United States, Australia, Japan, South Korea, Sweden, France, Spain and other global more than 2000 customers to provide high-quality products and services, has won the recognition and trust of customers in the industry.

More than 12 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

Mode of Transportation

By Air, fast but expensive.

By Sea, usual and economy.

By Train, suit for middle Asia countries.

FAQ

Does it support auditing?

Environmental Protection Science and Technology Park, Zhengfeng Road, High-tech Zone, Jinan City, Shandong Province

Whether to provide samples?

Free samples can be provided, and customers only need to pay the corresponding shipping costs

Delivery time

There are stock in the warehouse, and some products need to be customized for production within 3 working days, depending on the workshop situation and production schedule

What payment methods and currencies are accepted here?

We accept payment methods such as TT, Western Union, and letter of credit, etc. Meanwhile, we support currencies like US dollars, euros, RMB, and Bitcoin, etc.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Vivalr’s delivery rate is guaranteed to be 99.99%

2000+

square meter R&D center

12+

China(set) of R&D equipment

10+

Fume hood

20+

R&D team

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