Specialized inHigh Quality 4-Nitrophenyl alpha-D-galactopyranoside CAS 7493-95-0 With Good Priceand related product development and production

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High Quality 4-Nitrophenyl alpha-D-galactopyranoside (CAS 7493-95-0) User Guide

1. Product Information

Product Name: 4-Nitrophenyl alpha-D-galactopyranoside

CAS Number: 7493-95-0

Molecular Formula: C₁₂H₁₅NO₈

Molecular Weight: 301.25 g/mol

Appearance: White to off-white crystalline powder

Purity: ≥98% (HPLC)

Storage: Store at -20°C, protect from light and moisture.

2. Product Usage

4-Nitrophenyl alpha-D-galactopyranoside is a synthetic chromogenic substrate widely used in biochemical research and diagnostic applications. Its primary applications include:

Enzymatic assays for α-galactosidase activity.
Diagnostic kits for genetic disorders like Fabry disease.
Quality control in enzyme production and purification.
Research on carbohydrate metabolism and enzyme kinetics.

3. How to Use

Step-by-Step Protocol

Preparation: Dissolve in buffer (e.g., PBS or Tris-HCl) to a final concentration of 1-10 mM.
Sample Addition: Mix with enzyme solution (e.g., α-galactosidase) and incubate at 37°C for 15-60 minutes.
Reaction Stop: Add NaOH (0.1M) to terminate the reaction.
Detection: Measure absorbance at 405 nm using a spectrophotometer.

Note: Optimize incubation time and substrate concentration based on experimental conditions.

4. Detailed Use Cases

Case Study 1: Enzyme Kinetics Research

Background: A study aimed to characterize α-galactosidase activity in plant extracts.

Procedure:

Prepared 2 mM substrate solution in 50 mM sodium acetate buffer (pH 4.5).
Incubated with enzyme extract at 37°C for 30 minutes.
Measured kinetic parameters (K_m and V_max) using absorbance data.

Result: Successfully determined enzyme efficiency under varying pH conditions.

Case Study 2: Diagnostic Kit Development

Application: Used in a commercial kit for Fabry disease screening.

Workflow:

Integrated with patient serum samples and reaction buffer.
Automated absorbance reading enabled high-throughput screening.

Outcome: Achieved 99% specificity in detecting α-galactosidase deficiency.

5. Contact Information

For technical inquiries or bulk orders, contact us:

Email: info@vivalr.com
Phone: (86) 15866781826

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The Manager has Something to Say

This is William, CEO of Zhishang Chemical Co., Ltd.

Welcome to visit our factory . As the top chemical manufacturer, we have been striving for product quality, innovation , R&D, and customer service for the past 7 years. In the next 10 years or even longer, we are committed to becoming the most reliable chemical supplier in the world , creating a well-known international brand trusted by customers, with the spirit of “one meter wide, ten thousand meters deep”, we continue to focus on product research and development, continue to focus on customer service, continue to improve the supply chain service system, to create a professional chemical supply service team, to achieve win-win long-term cooperation. Please feel free to contact us if you have any questions.

Drug impurities to intermediates. Years of expertise.

Experience in the synthesis of complex compounds derived from American technology
Focus on the synthesis of difficult compounds
ISO 17034 dual system certification
National high-tech enterprise
2000 square R&D center
More than 20 research and development personnel,
12 sets of research and development equipment, 10 fume hoods and 6 university laboratories have been developed in cooperation
18 patents, 140 sets of chemical synthesis processes

Solid QMS. Offer top-quality products and services.

Through more than ten years of continuous technological innovation and experience accumulation, the team's R&D personnel have established a complete quality management system for CATO, and have successively obtained ISO9001, ISO17034, CNAS and other international certifications.
For the United States, Australia, Japan, South Korea, Sweden, France, Spain and other global more than 2000 customers to provide high-quality products and services, has won the recognition and trust of customers in the industry.

More than 12 sets of equipment to ensure quality.

The emphasis on quality is the basis for our survival and development, and is the most basic requirement for the products and services we provide. CATO has established a scientific, strict and comprehensive quality prevention and assurance system in accordance with international quality standards. Through advanced testing equipment such as GC-MS, chromatograph, nuclear magnetic resonance, scanning electron microscope, and ultraviolet light, quality management is implemented into every aspect of production and operation activities such as raw materials, inventory, production, testing, sales, verification, and training, and "All actions are controlled and traceable" as the work code of conduct for CATO employees.

Over 1000 cases. Synthesize complex compounds.

Currently, CATO serves more than 500 pharmaceutical companies, biotechnology and other companies around the world. Project types cover the entire cycle from pre-clinical to commercialization stages. As of 2020, CATO has delivered more than 1,000 projects in total.
The company has core technologies in key core technology fields such as high-end fluorine chemistry, asymmetric chiral synthesis, glycoside and biological enzyme technology, and continuous microreactors.

Mode of Transportation

By Air, fast but expensive.

By Sea, usual and economy.

By Train, suit for middle Asia countries.

FAQ

Does it support auditing?

Environmental Protection Science and Technology Park, Zhengfeng Road, High-tech Zone, Jinan City, Shandong Province

Whether to provide samples?

Free samples can be provided, and customers only need to pay the corresponding shipping costs

Delivery time

There are stock in the warehouse, and some products need to be customized for production within 3 working days, depending on the workshop situation and production schedule

What payment methods and currencies are accepted here?

We accept payment methods such as TT, Western Union, and letter of credit, etc. Meanwhile, we support currencies like US dollars, euros, RMB, and Bitcoin, etc.

Can additional test indicators be added?

We can provide moisture, UV, IR, HNMR, CNMR, two-dimensional nuclear magnetic spectroscopy, optical rotation detection, elemental analysis, TGA analysis and residue analysis and other related tests to support personalized needs.

Technical direction

In the early stage, our company mainly engaged in drug impurity synthesis and standard product production, with core technologies in chirality, fluorine chemistry, and heterocycles.

Why Vivalr’s delivery rate is guaranteed to be 99.99%

2000+

square meter R&D center

12+

China(set) of R&D equipment

10+

Fume hood

20+

R&D team

User’evaluation of us

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