Factory Supply TAPS CAS 29915-38-6 With Low Price: Comprehensive User Guide
Table of Contents
1. Product Information
Product Name: TAPS (CAS 29915-38-6)
Chemical Name: Tris(hydroxymethyl)methylaminopropanesulfonic acid
Molecular Formula: C7H17NO6S
Molecular Weight: 243.28 g/mol
Appearance: White crystalline powder
Purity: ≥99%
Storage: Room temperature, dry environment
2. Applications of TAPS
TAPS (CAS 29915-38-6) is a zwitterionic biological buffer widely used in:
- Biochemical research and molecular biology experiments
- Cell culture media preparation
- Diagnostic reagent formulations
- Pharmaceutical manufacturing
Its effective buffering range is pH 7.7–9.1, making it ideal for stabilizing pH in sensitive biological systems. TAPS also exhibits low binding affinity to metal ions, ensuring minimal interference in enzymatic reactions.
3. Usage Guidelines
Preparation Steps:
- Dissolve TAPS in distilled water at a concentration of 0.1–1.0 M.
- Adjust pH using NaOH or HCl as required.
- Filter-sterilize the solution (0.22 µm filter) for cell culture applications.
Precautions:
- Store in a tightly sealed container to avoid moisture absorption.
- Wear protective gloves and goggles during handling.
- Test compatibility with other compounds before large-scale use.
4. Detailed Use Cases
Case 1: Cell Culture Media Preparation
Step 1: Dissolve 22.33 g of TAPS in 800 mL distilled water.
Step 2: Adjust pH to 8.5 with 1M NaOH.
Step 3: Sterilize by filtration and add to DMEM medium at 25 mM final concentration.
Outcome: Stable pH maintenance during mammalian cell growth.
Case 2: Diagnostic Reagent Formulation
Step 1: Prepare 0.5M TAPS solution (pH 9.0).
Step 2: Mix with enzyme substrates and stabilizers.
Outcome: Enhanced accuracy in alkaline phosphatase-based assays.
5. Contact Information
For bulk orders or technical support:
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